斜纹夜蛾抗性种群中酶抑制剂对杀虫剂增效作用_英文_.pdfVIP

Acta Entomologica Sinica, June 2009, 52( 6) : 631- 639 ISSN 0454-6296 Synergism of insecticides by enzyme inhibitors in the resistant populations ofSp odop tera litura (Lepidoptera: Noctuidae) Mushta AHMAD ( Plant Protection D ivision, Nuclear Institute for Agriculture and Biology, Faisalabad 38000, Pakistan) : Abstract The effect of enzyme inhibitors piperonyl butoxide ( PBO) and tribufos ( DEF ) w as stud ied in combination w ith insecticides profenofos, methomy,l thiodicarb, cypermethrin, -cyhalothrin, bifenthrin, indoxacarb, and spinosad in the resistant Pakistani populations of Spodop tera litura using a leaf-d ip b ioassay. Both the inhib itors synergised carbamates methomyl and thiodicarb but show ed no synergistic effect on an organophosphate profenofos. These inhibitors produced a synergism w ith cypermethrin but had no synergism w ith b ifenthrin. PBO and DEF enhanced the toxicity of -cyhalothrin and indoxacarb in one popu lation but not in the other. Sp inosad was synergised by DEF but not by PBO. The potent synergism of carbamates, pyrethroids, indoxacarb and spinosad by PBO and DEF in the present study indicates that detoxification by cytochrome P450 monooxygenases and esterases is at least partially involved in mi parting resistance to these insecticides in S. litura. How ever, a lmi ited synergism of insecticides shown by both the synergists mi plies that othermechan isms such as target site insensitivity and reduced cuticular penetration may be more mi portant mechanisms of resistance in the Pak istani populations ofS. litura. : Key words Sp odop tera litura; insecticides; resistance; synergism; p iperonyl butox