细胞外微RNA_一种新型肺癌分子生物标志物.pdfVIP

HEREDITAS (Beijing) 2012 6 , 34(6): 651―658 ISSN 0253-9772 综 述 DOI: 10.3724/SP.J.1005.2012.00651 细胞外微RNA ：一种新型的肺癌分子生物标志物 杨丽华, 董琢, 龚朝辉 , 315211 尽管癌症的早期诊断和治疗在不断发展和进步, 但寻找一种敏感、准确和微创的分子生物标志物, 用于 肿瘤的诊断仍是一项艰巨任务。微RNA(miRNA)是一类长约21 ～24 个核甘酸的内源性非编码小分子RNA 。细 胞外 miRNA 作为一种新型的分子生物标志物, 在癌症诊断方面具有微创、高灵敏度和高特异性等许多潜在特 征。近年来, 细胞外miRNA 研究成果颇丰。文章就细胞外miRNA 的来源、功能、检测以及作为分子标志物在 肺癌诊断中的作用和目前存在的一些问题进行了综述。 细胞外微RNA; 分子生物标志物; 微创; 肺癌 Extracellular miRNA: a novel molecular biomarker for lung cancer YANG Li-Hua, DONG Zhuo, GONG Zhao-Hui Institute of Biochemistry and Molecular Biology , School of Medicine, Ningbo University, Ningbo 315211, China Abstract: Though continuous development and progress have been made in the early diagnosis and treatment of cancer, it is still difficult to find a sensitive, accurate and minimally invasive biomarker for cancer diagnosis and treatment. Mi- croRNA (miRNA) is a class of non-coding small endogenous RNAs of 21-24 nucleotides in length. As a novel molecular biomarker, extracellular miRNA (ec-miRNA) has the potential to be a minimally invasive, highly sensitive and highly spe- cific marker in cancer diagnosis. Many research achievements of ec-miRNA have been accumulated in recent years. In this paper, the origin, function and detection of ec-miRNA, its role in lung cancer diagnosis as a novel molecular biomarker, and some issues are reviewed. Keywords: extracellular microRNA; molecular biomarker; minimally invasive; lung cancer , (Small cell lung cancer, , SCLC NSCLC 5 SCLC)(Non-small cell lung cancer, 6% 17%[1] , NSCLC) 收稿日期: 2012−01−12; 修回日期: 2012−03−09 基金项目: (Y12C060009),