转化生长因子β1与基质金属蛋白酶2在多发病灶乳头状甲状腺癌中的表达及意义.docVIP

转化生长因子β1和基质金属蛋白酶 2在多发病灶乳头状甲状腺癌中的表达及意义 　　作者：袁宗怀　陈希平　张爱莉　王刚平 作者单位：276826　山东省，日照市人民医院普外二科(袁宗怀)，内科(陈希平、张爱莉)，病理科(王刚平) 　　【摘要】 目的　探讨转化生长因子Β1(TGF-Β1)和基质金属蛋白酶2(MMP-2)在多发病灶乳头状甲状腺癌(PTC)中的表达及意义。方法　应用免疫组化S-P法检测47例多灶性PTC中TGF-Β1和MMP-2的表达，以癌旁组织及单灶PTC作对照，比较TGF-Β1和MMP-2的阳性表达与多灶性PTC侵袭转移、复发等因素的关系及两者表达的相关性。结果　TGF-Β1和MMP-2在多灶性PTC中的表达均高于单灶PTC和癌旁组织，与多灶性PTC腺外浸润、淋巴结转移、肿瘤复发有关(P0.05)，与性别、年龄、家族史、是否是微小癌、多灶性PTC中病灶数目无关(P0.05);TGF-Β1和MMP-2二者表达呈正相关(r=0.920，P=0.000)。结论　TGF-Β1和MMP-2在多灶性PTC中阳性表达率高，与发生侵袭、转移和复发密切相关，两者可作为判断PTC生物学行为的指标。 　　【关键词】 甲状腺肿瘤;　癌，乳头状;　转化生长因子 Β1;　基质金属蛋白酶 2; 　　【Abstract】　Objective　To study the expression and clinical significance of TGF-Β1 and MMP-2 in mulitifocal papillary thyroid carcinoma (PTC).Methods　Immunohistochemical S-P method was used to investigate the expression of TGF-Β1 and MMP-2 in 47 cases of mulitifocal PTC and adjacent tissues.The expression of TGF-Β1 and MMP-2 and the risk factors were analyzed between solitary PTC and multifocal PTC group.Results　There were significant difference between mulitifocal thyroid cancer tissues，single focal PTCs and the adjacent tissue (P0.05).Overexpressions of TGF-Β1 and MMP-2 in mulitifocal PTCs were related to the degree of the infiltration，lymph node metastasis and tumor recurrence(P0.05).The expression of TGF-Β1 was correlated positively with MMP-2 (r=0.920，P=0.000).There was significant difference in expressions of TGF-Β1 and MMP-2 between mulitifocal and single focal PTCs in lymph node metastasis and tumor recurrence groups (P0.05).Conclusions　The high expressions of TGF-Β1 and MMP-2 in mulitifocal PTCs relate to the development，invasion，metastasis and recurrence.TGF-Β1 and MMP-2 might be considered as biologic behavior indicators of mulitifocal PTC. 　　【Key words】　Thyroid neoplasms;　Carcinoma，papillary;　Transforming growth factor beta1 1;　Matrix metalloproteinase 2 　　转化生长因子Β1(TGF-Β1)是一个多功能多肽链，能促进肿瘤转化和血管生成[1]。基质金属蛋白酶 2(MMP-2)是细胞外基质Ⅳ型胶原降解的关键酶，促进肿瘤细胞的浸润生长和转移[2]。乳头状甲状腺癌(papillary thyroid carcinoma，PTC)往往病灶多发，易侵袭、