Enzymatic Hydrolysis of Defatted Mackerel Protein with Low Bitter Taste.pdfVIP

Enzymatic Hydrolysis of Defatted Mackerel Protein with Low Bitter Taste.pdf

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Enzymatic Hydrolysis of Defatted Mackerel Protein with Low Bitter Taste.pdf

d.OceanUniv.China OceanicandCoastalSeaResearch D0I10.1007/s11802—0l1一l785—6 ISSN 1672—5182,201110 1 :85—92 http://~,wwOUCedu.cn/x wb/ E—mailxb3’n’。6@OIIC.educn EnzymaticHydrolysisofDefa LowBitterTaste HOU Hu,LIBafang,andZHAO Xue CollegeofFoodienceandEngineering,OceanUniversity0fChinaQingdao266003,P.R.China ReceivedJune30,2010;revisedSeptember21,2010;acceptedSeptember29,2010 ⑥ OceanUniversityofChina。SciencePressandSpring-VerlagBerlinHeidelberg2010 Abstract Ultrasound—assistedsolventextractionwasconfirmedasanove1.effectivemethodforseparatinglipidfrom mackerelpro— tein.resultinginadegreasingrate DR of95%andanitrogenFCCOVCry NR of88.6%.Toobtainproteinhydrolysateswithhighnl— trogenrecoveryandlow bittertaste.enzymatichydrolysiswasperformedusingeightcommerciallyavailableproteases.1tturnedout thattheoptimum enzymewasthe M‘ixedenzymesforanimalproteolysis’.Anenzymedosageof4%.atemperatureof50~C,anda hydro1ysistimeof300rainwerefoundtobetheoptimum conditionstoobtainhighNR 84-28% anddegreeofhydrolysis DH, 16.18%1byorthogonalexperimentsGlutamicacidwasthemostabundantaminoacidofMDP defattedmackerelprotein andMDPH fdefattedmackere1proteinhydrolysates .ComparedwiththeFAO/WHOreferenceprotein,theessentiaIaminoacidchemicalscores fCS1weregreaterthanI.0 1.0一1.7 inMDPH,whichisreflectiveofhighnutritionalvalue.This,coupledwiththelightcolorand slightfishyodor.indicatesthatMDPH wouldpotentiallyhaveawiderangeofapplicationssuchasnutritionaladditives,functional ingredients,andsoon. Keywords mackerel;hydrolysis;ultrasound;defatted;bitter;aminoacidcomposition dependslargelyon itsaftcontentand itisnecessaryto removeaftrfom thefishofrqualityproteinproducts. 1IntrOductiOn Additionally.much efofrthasbeen made to utilize Accordingtothestatistics.40%一50% ofthetota1fish mackere1assmoked,canned,chilled,rfozen,andrestruc— catchintheworldaredarkmusclefishspecies Hultin utredfishproducts Losadaetal,2005 .Hydrolysisof andKelleher2000 .Duetothedeclinedfishresources, proteinwithproteolyticenzymesca

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