沙苑子黄酮对人肝癌HepG2细胞体外抑制作用_杜崇民.pdfVIP

# 900# 辽宁中医杂志 2010年第 37 卷第 5期 H epG2 1 1 2 , , , ( 11 苏州大学药学院, 江苏 苏州 215123; 21 苏州中药研究所, 江苏 苏州 2 15 123) : 目的: 观察沙苑子黄酮对人肝癌H epG 2 细胞的体外抑制作用方法: 采用MTT 法观察沙苑子黄酮对人肝癌 H epG2 细胞的抑制作用, 进一步观察沙苑子黄酮对H epG 2 细胞形态超微 构生长曲线以及集落形成的影响 果: 沙苑子黄酮可明显抑制 H epG 2肿瘤细胞的增殖和集落的形成, 50L g /mL 剂量组集落形成率为 251 6( P 010 1), 远低于 对照组; 光镜及透射电镜可以发现细胞形态有凋亡特征变化 论: 沙苑子黄酮体外对人肝癌 H epG 2 细胞具有明显的 抑制肿瘤细胞增殖的作用, 其作用与诱导肿瘤细胞凋亡有关 : 沙苑子黄酮; H epG 2; 抗肿瘤 : R 28515 : A : 1000- 1719( 20 10) 05- 0900- 03 Inhibiting Effect of Flavonoids ofA s trag a lus Comp lana tus on H uman H epatoma Carcinom a CellH epG2 in Vitro 1 1 1 2 D U Chong-m in , LIU Chun-yu , W U W en-Qian , GU Zhen-lun ( 11Co llege of Pharmacy, M ed ical Co llege of Soochow Uni ersity; Suzhou 215 123, J iangsu China; 21Suzhou Institute of ChineseM eter iaM ed ica, Soochow Uni ersity; Suzhou 2 15123, J iang su China) Abstract: Obj ecti e: T o obse v e the inh ib iting effect of flavono ids ofA stragalus comp lanatus( FAC ) on hum an hepatoma ca c-i nom a ce llH epG 2. M ethods: T he inhibiting effect on hum an hepatom a ca c inom a ce ll H epG2 w as obse ved by MTT, g ow th cu v e assay and co lony fo m ation assay. Its effec t on m o pho logy and u lt am ic ost uctu e ofH epG2 was obse ved by light m ic o scope and t ansm ission e lect on m ic oscope. R esults: T he colony fo m ation atio is low com pa ed w ith cont o l g oup(P 01 01), co lony fo - m ation atio of FAC- d ( 50L g/mL) is 2516% ; apoptotic cha ac te istic could be find by light m ic oscope and t ansm ission e lec- t on m ic oscope. Conclusion: FAC