噻唑蓝法测定抗肿瘤复方制剂仙慈丹体外抗肿瘤活性.pdfVIP

# # 1 1, 2* 1 1 1 2 1, 2 罗明琍 , 黄松 , 王杰 , 丁婕 , 黄鸣清 , 赵爱国 , 赖小平 ( 11 , 5 10006 21 , 523808) : 分离筛选出抗肿瘤复方制剂仙慈丹( XCD ) 抗肿瘤活性部位 采用大孔树脂柱色谱法对抗肿瘤复方制 剂 XCD 水提取液进行分段富集, 运用噻唑蓝(M TT ) 比色法对分段富集得到 部位进行体外抗肿瘤活性测定 抗肿瘤 复方制剂 XCD 水提取液过 A B-8型树脂纯化得到 P2 ( 体积分数 20% 乙醇洗脱液) P3 (体积分数 4 0% 乙醇洗脱液) P4 (体 积分数 60% 乙醇洗脱液) P5 (体积分数 80% 乙醇洗脱液) 对人肝癌H epG 2 细胞表现出较强 抑制作用 XCD 水提液 过大孔树脂后乙醇洗脱部位是XCD 制剂 抗肿瘤活性部位 : 噻唑蓝 复方制剂 体外 抗肿瘤活性 仙慈丹 : R 961 : A : 1001- 2494( 2010) 23- 18 16- 0 3 Determination ofAnt-iTum orActivity of Compound Preparation Xiancidan in Viro byMTTM ethod 1 1, 2* 1 1 1 2 1, 2 LUO M ing- li, HUANG Song , WANG Jie , D ING J ie , HUANG M ing-qing , ZHAO A -i guo , LA I X iao-p ing ( 11 Guangzhou University of ChineseM edicine, Guangzhou 51000 6, China; 21 D ongguan Institutionf orM athematics and Theoretics En- g ineering Research, D ongg uan 52 380 8, Ch ina ABSTRACT: OBJ CTIV T o separate and se lect ant-i tum or ac tiv ity site of the anti-tum or compound prepa ra tion X iancidan ( XCD ) 1 M THODS The m ean s o f m acroporou s resin column chrom atography( A B-8) w as u sed to sep arate the w ater extract from Chinese drug XCD1 T he ant-i tum or activ ities of the d ifferen t extrac ts in v itro w ere ana lyzed w ith MTT m e thod1 R SULTS P2 ( 20% e th ano l e- lu tion) , P3( 4 0% eth ano l e lution ) , P4 ( 60% eth ano l elution) and P5 ( 80% e th ano l e lution) strong ly inhib ited cell pro lifera tion of H epG 21 CONCLUSION Eth anol e lution po sition is the anti-tum or ex tract from XCD 1 K Y WORDS: M TT com pound prep aration in v itro ant-i tumo r ac tiv ity X ianc idan ( XCD )