瘦素预处理对小鼠心肌冷热缺血再灌注损伤差异性.pdfVIP

生围耋芏堂苤盍!!!；至!!爿箜!!鲞 瘦素预处理对小鼠心肌冷／热缺血再灌注损伤的差异性 吕祥威 徐彤彤 武 琦 覃 泱 (桂林医学院附属医院特需病区，广西 桂林541001) [摘要] 鼠被随机分为3组：(1)心肌冷IRI组：瘦素预处理(术前30 h收获标本，观察组织形态学变化，检测小鼠移 4℃uw液中保存6h；(2)心肌热IRI组；(3)心肌缺血再灌注模型对照组(MIRI组)。分别于术后6 植心脏及血清中性粒细胞活性，炎性细胞因子水平等。结果 (MPO)活性、血清细胞炎性因子肿瘤坏死因子(TNF)。d，白介素(IL)一l 冷／热IRI，减轻炎症反应，但是小鼠心肌冷／热IRI之间的差异性无统计学意义。 (关键词]瘦素预处理；心肌冷／热缺血再灌注损伤；心脏移植；炎性细胞因子 [中图分类号]R541．4[文献标识码]A research oncoId／hot inmice Differences injury reperfIlsion ofleptinpretr呀tment myocardialischemia Lii Qi，甜口厶 Xiang-Wei，XUTong-Tong，WU of Medical TheVIP Guilin College，Guilin541001，G岫n弘i，China Ward，A胤iatedHospital of oncold／hot ischemia thedifbrencesthe objective‘％deteet 【Abstraet】 leptinpretreatment myocardialreperfusion were a cardiac model．Methods heart were in30maleC57BL／6miceand mn— murine RI)in tmnsplant Syngeneictransplantsped、omed domizedinto3 in Ns be— gmup，leptin50斗g／kg i11jected pretreatment 200斗l groups：q)cardiactransplan‘group；②1eptin fore about30min wereretrievedat6hafter andsenIm before gr“ts transplantation(at repedhsion)；③MIRIgroup．The reperfusion．Gr—ts collectedfor and toassess iIlflammation．ResultsThe were