高效液相色谱法对泡菜中L_乳酸与D_乳酸的手性分离与测定.pdfVIP

Modern Food Science and Technology Vol.23 No.8(98) L-D-8000 r/min 20 min0.45 µm 254 nm Chirex 3126 (D)-penicillami 4.6 mm i.d×250 mm L, 5 µm 2 mmol/L CuSO4 5 %L- D-2.5-20.0 g/L 0.999L-D-99.82 %100.02 %L-D- L-D- TS255.54A :1673-9078(2007)08-0074-03 Chiral Separation and Determination of L-and D-lactic Acid in Pickles by High-performance Liquid Chromatoraphy 1 1 1 1 2 2 LIU Dong-mei , WU Hui , YU Yi-gang , LI Xiao-feng , GUO Wen-jie , ZENG Wei-jie 1.College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China 2.Guangzhou FLM Scientific Instrument Co., Ltd, Guangzhou 510620, China Abstract: A new method for the separation and determination of L- and D-lactic acid in pickles supernatants by high-performance liquid chromatography (HPLC) with a chiral colomn was studied here. The culture supernatants of pickles were centrifuged for 20 min at 8000 r/min and the supernatant fractions were filtered through a 0.45 µm membrane. The samples were then analyzed by HPLC on chiral columns with a PDA detector at 254 nm. High separation efficiency was achieved by using a Chirex 3126 (D)-penicillami column (4.6 mm ID×250 mm L, 5 µm) and a mobile phase consisting of CuSO4 (2 mmol/L) and isopropyl alcohol-water (5:95). The calibration curves of L- and R- lactic acid were linear with regressions being of 0.999 in the concentration range of 2.5-20.0 g/L. The average recovery was 99.82 % for D--lactic acid and 100.02 % for L-lactic acid in filtrate of pickles. The method was simple and easy to operate with high accuracy and reproducibility and it was suitable for detection the concentration of L- and D-