Matrilin-1基因多态性对青少年特发性脊柱侧凸进展预测价值.pdfVIP

·基础研究· Matrilin一1基因多态性对青少年特发陛 脊柱侧凸进展的预测价值 陈志军 邱勇 王斌 俞杨 朱泽 【摘要】 目的探讨Matrilin一1基因多态性预测青少年特发性脊柱侧凸进展成为严重脊柱侧凸的 价值。方法研究对象为267例仅随访或手术治疗的AIS患者．记录末次随访或术前最大Cobb角、月 经初潮年龄、侧凸弯型、身高、臂长、体重。选取Matrilin一1基因启动子区域位点rsll49048进行PCR— RFLP基因分型。进展型侧凸(侧凸进展成为严重脊柱侧凸)定义为骨骼发育未成熟时，Cobb角40。或骨 骼发育成熟时，Cobb角500。AIS患者按照侧凸进展型与非进展型分为两组。比较两组间的临床特征及 基因型分布的差异。分析侧凸进展的危险因素。结果267例AIS患者有162例(60．7％)为非进展型， 105例(39．3％)为进展型。月经初潮年龄晚、三弯及胸腰双主弯、基因型GG为侧凸进展的危险因素。结 论Matrilin—i基因多态性可用来预测青少年特发性脊柱侧凸进展．结合其他预测因素可提高预测的 准确率。 【关键词】脊柱侧凸；基因多态性；进展；预测；Matrilin一1基因 Matrilin-1 ofadolescent seoliosis gene(MATNl)polymorphismpredictsprogression Idiopathic CHEN Bin,et DrumTower Zhi-jun,QIUYong,WANGa1．印ineSurgery,theAffiliated HospitalofMedical University,Nanjing210008，China School,Nanjing To thevalueofmatrilin-1 to which 【Abstract】0bjective investigate genepolymorphismspredict are to toaseverescoliosis．Methods7Ihis consistedof267 patientslikelyprogress studypopulation pa— tientswithAISwhich receivedobservationor maximumCobb at only surgery．The angle，agemenarche， curve a．rm wererecorded．Thersl149048 in ofmatrilin一1 pattern，height，andspan polymorphismpromoter WaSchoosedfor PCR—RFLP toaseverecurvewasdefinedusual gene genotypingby method．Progression per clinical toa40。curveinanindividualstill or toa50。curvein criteria(progression growingprogression an were and ofclin- adult)．Allpatientscategorizedbyprogressivenon-progressivegroups．Andcomparisons distribution Of icalcharacteristicand betweenthesetwo were the267 genotype