强直性脊柱炎患者血清和关节滑膜液IL-8、IP-10及RANTES水平测定及其临床意义.pdfVIP

维普资讯 逝 ()7生鱼 鲞第 · 1149 · 强直性脊柱炎患者血清与关节滑膜液 IL一8、IP一1O及 RANTES水平测定及其临 床意义 张颖 吴华香 朱永 良 【摘要】 目的 探讨 IL一8、 干扰素诱生蛋白10(IP—l0)及活化正常T细胞表达和分泌的涧节物(RAN3ES) 与强直性脊柱炎(AS)患者病情的关系。方法 采用ELISA法检测 31例活动期As患者(观察组)血清 、30名健康者 f对照组)血清。观察组又按临床症状分为周围型AS(13例)和叶1轴型AS(18例)，检测周围型As患者关节液IL一8、 IP一10和RANTES的水平。结果 观察组血清 IL一8、IP一10和RANTES水平均高于对照组(均PO．05)。AS患者关 节滑膜液中IL～8和 IP一1O水平高于血清水平(P0．05)；而RANTES水平低于血清水平(R0．05)。周罔型As患者血 清 IL～8水平较中轴型As患者 著增高 (PO．O1)。血清 RANTES水平与Bath强直性脊柱炎活动指标 (BASDAI) 呈正相关 (r=0．575，P0．05)。结论 II一8血清水平可能与As患者发病形式有关 ，RANTES可作为病情活动性指 标。 【关键词 】强直性脊柱炎 IL一8 f扰素诱生蛋白 活化正常T细胞表达和分泌的调节物 IL-8，IP-IOandRANTESinserum andsynovialfluidfrom patientswith ankylosingspondylitis ZHANG ring, WUHuaxiwzg,ZHUYonglicmg． Department Rheumatology,2ndA所limedHospital,zhejiangU}liversilySchoolof Medicine，Hangzhou310009,China A【bstract】Objective T0investigatethelevelsofII一8，IP一10andRANTESinserulHandsynovialfluidfrompa— tientswithankylosingspondylitis，andtheirelinical significance．Methods thelevelsofIL～8．IP-IOandRANTESin SeFUlllfrom31patientswithankyofsingspondylitis (experimentgroupJand30healthysubjects (eontrolrgoup)，andthosein synovialfluidfrom 13patientswithankylosingspondylitisweredeterminedhyELISAmethods．Experimentgroupweresuh- divi(tedintoperiphera1typeandcentralaxialtypeaccordingtodif1)rentclinicalmanifestation．Results Serum levelsof II 8．IP-IOandRANFES inpatientswithankylosingspondylitisweresignificantlyhigherthanthoseinheahhycontrol group(PO．05)．Inankyofsingspomlylitispatientsgroup，thelevelsofIL-8，IP-IOinsynovialfluidweresignificantlyhigher thanthoseinSerllm，whilethelevelofRANTESinsynovialfhlidwassignificantlylowerthanthatinSeFlllll(PO．05)．Sennn levelofIL一8inpatientswithperipheral typeofankylosingspondylitiswashigherthanthatinpatientswithcentralaxialtype (PO．01)．Therewas positivecorr