柱切换高效液相色谱法和其在食品检验中的应用.pdfVIP

156 2009 21 2 CHINESE JOURNAL OF FOOD HYGIENE 综述 赵 凯 杨大进 王竹天 ( 中国疾病预防控制中心营养与食品安全所, 北京 100021) : 柱切换是指利用多通路切换阀改变进样阀与色谱柱色谱柱与色谱柱色谱柱与检测器之间的 接关系 的技术对于样品中多组分待测物的净化和分离分析, 柱切换高效液相色谱法是有效的分析方法样品前处理后 进样, 待测物可在线进行富集净化衍生化等, 从而减少有机溶剂的用量提高回收率降低检出限和定量限由 此综述柱切换的原理和装置, 在食品中农药残留检测兽药残留检测食品成分分析和其它成分的应用, 以及存在 的问题 : 色谱法, 高效液相; 食品分析; 分离提纯; 柱切换 ColumnSwitching High Performance Liquid Chromatography and Its Application to Food Inspection ZHAO Kai, YANG Dajin, WANG Zhutian (Nat ional Inst itute fo Nut ition and Food Safety, Chinese CDC, Beijing 100021, China) Abstract: The technique of columnswitching could alte the connections between inj ection valve and column, column and column, column and detecto . The columnswitching high pe fo mance liquid ch omatog aphy was a powe ful technique fo the sepa ation and cleanup of multicomponent mixtu es. The samples we e di ectly injected afte simple p et eatment . Analytes of inte est we e concent ated, cleaned and de ivated online, and so on. The method could dec ease o ganic solvent volume, imp ove ecove y and lowe detect limit and quantification limit . The columnswitching high pe fo mance liquid ch omatog aphy was widely used in d ug kinetic esea ch, envi onmental su veillance, et al . The p inciple and installation of columnswitching technique, application to pesticide and vete ina y d ug esidue dete minations, nut ients analysis in food we e eviewed. Key word: Ch omatog aphy ,High P essu e Liquid; Food Analysis; Isolation and Pu ification; Columnswitching, HPLC 1 , ( columnswitching)