SSR分子标记的开发策略概述.pdfVIP

27 4 ( ) V o.l 27 N o. 4 2009 12 Journal ofHubeiU niversity fo r N ationalities(N atura l Science Ed ition) D ec. 2009 SSR 1, 2 1, 2 1, 2 , , ( 1. 中国地质大学 生态环境研究所; 湖北武汉 430074; 2. 中国地质大学 生物地质与环境地质教育部重点实验室, 湖北武汉 430074) : SSR 分子标记 一种基于 DNA 重复序列长度多态性的分子标记技术, 进行群体遗传学结构分析构建 遗传连锁图谱非常有力的工具, 应用该技术的前提条件 要有相应的 SSR 引物. 综述了几种有代表性的 SSR 引物 开发策略, 旨在为各物种 SSR标记的开发提供参考. : SSR; 分子标记; 策略 : Q 78 : A : 1008- 8423( 2009) 04- 0462- 06 Strategies for D evelop ing SSR M olecu lar M arkers 1, 2 1, 2 1, 2 O rgil , J A X iao , GE T a i- m ing ( 1. nstitute of Ecology Env ironm en tal Science, Ch ina U n ive rs ity of G eosc iences, W uhan 430074, Ch ina; 2. K ey L aboratory of B iogeo logy and Env ironm enta lG eo logy ofM inistry of Educa tion, China U niversity of G eosciences, W uhan 430074, Ch ina) Abs tract: SSR is one of m olecular m arkers based on DNA leng th po lym orphism and a pow erful too l for popu lation genetic stud ies and prmi ary gene tic linkage m aps construct ion. The m ajor draw back of SSRs is that, for the f irst u tilizat ion in a new spec ies, they need to be iso lated in order to synthesize the the prmi - ers. The a mi of th is paper is to review the severa l representa tive m ethods of SSR prmi ers isolat ion de- scribed in the literature and to prov ide usefu l inform ation in m ak ing appropriate choices am ong the large num ber of currently ava ilab le opt ions. K ey w ord s: SSR; m olecu lar m arker; strategy