黄芪、三七不同剂量配伍对肾小球系膜细胞增殖的影响.docVIP

黄芪、三七不同剂量配伍对肾小球系膜细胞增殖的影响 　　作者：刘明平，黄兆胜，吴庆光，韦品清 作者单位：广州中医药大学：1. 中药学院;2. 护理学院，广东广州 510405 　　【摘要】 目的 通过考察通脉口服液方中黄芪、三七不同剂量配伍对肾小球系膜细胞增殖的影响，筛选其最佳配伍剂量。方法 采用血清药理学方法，制备不同剂量配伍的通脉含药血清，体外检测其对肾小球系膜细胞(GMC)增殖的影响。结果 通脉口服液，黄芪、三七不同剂量配伍含药血清，无论在正常培养条件下还是在脂多糖(LPS)刺激条件下，均对GMC增殖有一定的抑制作用;各剂量配比组之间比较，通脉3∶1含药血清抑制作用优于通脉6∶1、1.5∶1含药血清。结论 原方黄芪、三七剂量按3∶1配比具有一定的合理性。 　　【关键词】 黄芪 三七 肾小球系膜细胞 配伍研究 　　Effect on proliferation of glomerular mesangial cell by the patible proportion between Radix Astrsgaliand Radix Notoginseng in 　Tongmai Oral Liquid 　　LIU Mingping, HUANG Zhaosheng, WU Qingguang, WEI Pinqing 　　1. College of Traditional Chinese Medicine; 2. College of Nursing Science,Guangzhou University of Chinese Medicine, Guangzhou 510405, China 　　ABSTRACT: Objective To study the effect on proliferation of glomerular mesangial (GMC) cell by the dose compatible proportion between Radix Astrsgali and Radix Notoginseng in Tongmai Oral Liquid (TMOL) so as to select the optimal dose proportion. Methods We used serological pharmacological methods to prepare Decoction of Tongmai of different dose proportions and assay its effect on the proliferation of GMC to confirm the best dose compatible proportion between Radix Astrsgali and Radix Notoginseng of Decoction of Tongmai. Results Both TMOL and the different proportions between Radix Astrsgali and Radix Notoginseng (6∶1, 3∶1, 1.5∶1) of Decoction of Tongmai could inhibit the proliferation of GMC cultured either in fetal cow serum (FCS) or in FCS plus lipoplysaccharide. The inhibitory effect of Decoction of Tongmai (3∶1) group was better than both Decoction of Tongmai 6∶1 and Decoction of Tongmai 1.5∶1. Conclusion The dose compatible proportion between Radix Astrsgali and Radix Notoginseng in TMOL was reasonable. 　　KEY WORDS: Radix Astrsgali; Radix Notoginseng; glomerular mesangial cell; study on compatible proportion 　　通脉口服液为治疗慢性肾炎的有效经验方，方中黄芪、三七剂量按3∶1经验使用。为进一步寻找其最佳配伍比例，本研究以肾小球系膜细胞(glomerular mesangial cell, GMC)增殖为药效学导向指标，对方中黄芪、三七的不