脑梗死小鼠凋亡神经元及脑血管内皮细胞超微结构的观察.pdfVIP

·128 · 200042 2 Chin J Geriatr Cardio asc Cerebro asc Dis Apr 2000 Vol 2 No 2 脑梗死小鼠凋亡神经元及脑血管内皮细胞超微结构的观察 李振光, 伍期专, 姚存姗 (, 　100034) :目的　观察脑梗死后不同时间点脑组织的病理变化, 尤其是凋 神经元及血管内皮细胞超微结构的改变。 方法　采用经皮光化学脑梗死模型, 氯代三苯基四氮唑(TTC)染色, 光镜及透射电镜观察。 结果　缺血24 ～ 48 h, 脑组织及血管内皮细胞损伤最严重, 缺血6 h 至1 个月均可见凋 神经元, 其中以48 h 最多见。 早期应用巴曲酶对 缺血脑组织及血管内皮细胞有保护作用。 结论　脑缺血后神经元在相当长的 一段时间内均可发生凋 。保护血 管内皮细胞、抑制神经元凋 对改善缺血脑组织的预后具有重要意义。 :脑梗塞;小鼠;细胞凋 :R 743.33　　　　　　:A　　　　　　:1009-0126(2000)02-0 128-04　　 Long-term observation on ultrastructure of apoptosis of neuron and endothelial cells of cerebral vessels in mice with photochemically induced cerebral infarction LI Zhen-guang,WU Qi-zhuan,YAO Cun-shan (Laboratory of Neurochemistry , First Aff iliated Hosp ital, Beij ing Medical University , Beij ing 　100034, China) Abstract:Objective　To in estigate the cerebral pathological changes at different time points after brain in- farction, especially the changes of the ultrastructure of apoptosis of neuron and ascular endothelium.Methods 　The transdermically photochemically induced cerebral infarction model was used.TTC(2, 3, 5-triphenyl- tetrazolium chloride )staining, light and transmission electron microscopy were chosen to in estigate the chang- es.Results　The damage of brain tissue and ascular endothelium was most prominent from 24 to 48 hours af- ter brain infarction .The apoptosis of neuron could be found from 6 hours to 1month after brain infarction, and was found most frequently at 48 hours.The administration of batroxobin protected the brain tissue and endothe- lium at the early stage of cerebral ischemia.Conclusion　The apoptosis of neuron could occur during a rather long period and the time window of inhibiting ischemic apoptosis is relati e long.The pathomorphism of isch-