2土壤食细菌线虫的分离和富集培养方法_李辉信.pdfVIP

2002, 25 ( 2) : 71~ 74 J ournal of Nanj ing Agricultural University 李辉信, 陈小云, 胡锋 ( , 2 10095) : B1 B2 , ( P rotorha bditis sp. ) , B1 ( Pseudomonas sp. ) , B2 ( A cetobacter sp. ) B1 B2 , , ; B1 B2 , , : ; ; : S15411 : A : 1000O2030 ( 2002) 02O0071O04 Isolation and enrichment culturing of a soil bacteria-l feeding nematode LI Hui-xin, CHEN Xi o-yun, HU Feng ( College of Resources nd Environment l Sciences, N njing Agric Univ, N njing 210095, Chin ) Abstract: A soil b cterivorous nem tode feeding on b cteri B1 nd B2 w s isol ted from rhizosphere soil of whe t nd cultured on pot to sucrose g r medium ( PSA) . The nem tode w s identified s Protorhabd it is sp. ; B1 nd B2 were identified s Pseudomonas sp. nd A cetobacter sp. , respectively. B1 nd B2 didn. t secrete polys cch rose on beef polypeptone g r medium ( BPA) nd the nem tode didn. t grow on BPA medium with B1 nd B2, while B1 nd B2 secreted polys cch rose on PSA medium. The nem tode grew very well on PSA medium with B1 nd B2. Polys cch rose constitutes n import nt extern l environment for nem tode growth. Key words: soil b cteri -l feeding nem tode; enrichment culturing; polys cch rose ( free-living nem todes) , [ 1~ 3] 60% ~ 80%, 90% , , , , , , [ 4~ 8] , , , 1 材料与方法 111 [ 9] B erm nn , , ( ) , , 24 h , , 20 min, 3 000 r#min- 15 min, : 2001O05O31 : ( : ( 1964) , , , E-m il: huixinli@ nj u. edu. cn # 72 # 25 , 5 , 3 000 r#min- 15 min, 011% 01002% 112 10- 6, 10- 7, 10- 81 mL , 25 e 3~ 5 d, (