tPA信号肽和猪繁殖与呼吸综合征病毒ORF5基因原核表达载体的构建.pdfVIP

# 58 # 2009 36 3 tPA ORF 5 , , , , , , ( , 450002) :GenBank( E00896) (tissue plasminogen activator,tP ) , Signal P3.0 Server tP 63 bp, 1 , PCR tP 1 , PCR tP OR F 5 , tP OR F 5 , GP5 :PRRSV;OR F 5 ; tP ; :Q78 : : 1671-7236(2009) 03-0058-05 ( porcine reproduc- ; , tive and respiratory syndrome virus, PRRSV) , ( nidovirales) ( arterivin- PRRSV dae) (arterivirus) , RN 15 kb, 8 , ,, ORF1aORF1bORF2~ 7(, 2001) , OR F5 (GP5) , , ,, (Hall,2005) , ,PRRSV LV (Sur , 1998;Delputte , 2002) ,,GP5 VR2332 ( Peter, 2004) , (porcine reproductive and respiratory syndrome, PRRSV ,PRRS PRRS) , GP5 DN , GP5 : 2008-08-07 , : ( 1982- ) , , , , : , : E-mail:xpa-88@ sohu. com (Pirzadeh , 1997) , Recent Progress in Zona-Free Somatic Cell Nuclear Transfer Y NG L-i jun, LI Yu (Key Laboratory of Mammal Reproductive Biology and Biotechnology, Ministry of Education, Inner M ongolia U niversity,Hohhot 010021, China) Abstract: t present, theways of somatic cell nuclear transfer( SCNT ) was still the same to the Willadsen. s method: enu- cleation, injection, fusion and activation. With its low efficiency and requiring sophisticated and expensive micromanipulation e- quipment and considerable skills from the operator, so attempt to simplify and establish a cloing process suitablefor manufactur- ing conditions,thereby improve the effieiency of nuclear transfer has been a tireless efforts of the people.T his paper introduces the methods of zona-free SCNT and the single embryo culture