一种新的体外细胞牵张刺激装置.pdfVIP

XI Yu-Tao et al: Centrifugal Force Stretcher a New of in vitro Mechanical Cell Stimulator 生理学报 Acta Physiologica Sinica, June 25, 2004, 56 (3): 419-423 419 Experimental Technique Centrifugal force stretcher a new of in vitro mechanical cell stimulator XI Yu-Tao, BAI Xiao-Jun, WU Ge-Ru, MA Ai-Qun* Department of Cardiology, the First Hospital, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi’an Jiaotong University , Xi’an 710061, China Abstract: A number of mechanical cell stimulators have been used to study the effect of mechanical stimulation on cells in vitro. But the efficiency of these devices is not fully desirable. We recently developed a new device for mechanical cell stimulation, the centrifugal force stretcher, and compared its efficacy with that of the traditional Flexercell Strain Unit. When the mechanical stretcher circumrotates with certain speed, cardiac myocytes attached on the plate are stretched and elongated by centrifugal force. Neonatal rat cardiac myocytes were isolated by enzymatic dissociation from the hearts of 3~5 d old Sprague Dawley rats, and were mechanically stimulated by traditional 20% stretch and 180 r/min centrifugal force for 12 and 24 h. The effects of mechanical stimulation on the hypertrophic response of neonatal rat cardiac myocytes and production of angiotensin Ⅱ (Ang Ⅱ) were examined. Compared with the non-stretch group, the radioactivity of 3H-leucine incorporated into the stretch-stimulated cardiac myocytes in the centrifugal force stretch group was significantly higher [(1295.17 ±51.19) vs (1122.67 ±51.63) in 12 h; (1447.5 ±35.96) vs (1210.67 ±90.92) in 24 h, P0.05]. Ang Ⅱ was also dramatically increased by 128% in 12 h (P0.05) and 139% in 24 h (P0.01). After the myocytes was stretched for 24 h, the LDH level in the medium in the Flexercell Strain Unit group was significantly high