乌司他丁对心肺复苏后大鼠脑组织含水量及CD11b的影响.pdfVIP

2009 年6 月第47 卷第17 期 ·基础研究· 乌司他丁对心肺复苏后大鼠脑组织含水量及CD11b 的影响 1 2* 2 2 2 周晓峰 王 晶 赵 妍 邢绣荣 秦 俭 （1.首都医科大学大兴医院急诊科，北京102600；2.首都医科大学宣武医院急诊科，北京100053） [摘要] 目的 探讨乌司他丁对心肺复苏早期大鼠脑组织含水量及脑组织CD11b 水平的影响。方法 将30 只SD 大鼠随机分 为假手术对照组、心肺复苏（CPR）组、乌司他丁组，每组各10 只大鼠。于自主呼吸循环恢复后2h ，采用干湿法检测各组大鼠 脑组织含水量，采用ELISE 法检测大鼠脑组织CD11b 水平。结果 ①脑组织含水量：CPR 组为（80.4±2.0）% ，较对照组 （76.7±1.3）%显著增加（P ＜0.01）；乌司他丁组（77.6±1.5）%，较CPR 组显著减少（P ＜0.01）。②脑组织CD11b 含量：CPR 组 为（620.488±38.723）ng/mg，较对照组（453.189±121.403）ng/mg 明显增高（P ＜0.01）；乌司他丁组（476.875±114.686）ng/mg， 较CPR 组显著减少（P ＜0.05）。结论 CPR 早期即可发生脑水肿，脑组织CD11b 水平显著增加；应用乌司他丁能有效降低脑 组织含水量和脑组织CD11b 水平。 [关键词] 心肺复苏；缺血再灌注损伤；脑水肿；CD11b ；乌司他丁 [中图分类号] R392 [文献标识码] A [文章编号] 1673- 9701（2009）17- 25- 03 The Effect s of UTI on Brain Edema and CD11b in Brains of Rat s aft er CPR ZHOU Xiaofeng1 WANG Jing2 ZHAO Yan2 XING Xiurong2 QIN Jian2 1.Department of Emergency，Daxing Hospital ，Beijing 102600 ；2.Xuanwu Hospital ，Capital Medical University，Beijing 100053 [Abst ract ] Object ive To observe the effects of UTI on the moisture capacity and CD11b of brains in CPR rats. Met hods 30 grown male Sprague Dawley（SD）rats were divided randomly into three groups ：control group ，resuscitation group and UTI group. Each group had 10 rats. The last two groups were used to make CPR models induced by asphyxia and were observed lasting 2 hours after ROSC. Then all rats were caused dead and brain tissues were took out. Brain tissues were weighed （humid weight），then they were dried in a baker and scaled dry weight. Moisture capacities were calculated by Elliott method. The lever of CD11b in brain tissue was determined by ELISA method. Result s ①The