草莓根围拮抗细菌的多样性分析及其田间应用.pdfVIP

25( 1) 52- 57 Chinese Journal of Biological Control 2009 7 草莓根围拮抗细菌的多样性分析及其田间应用 * 杨敬辉, 朱桂梅, 陈宏州, 潘以楼 , 吴琴燕 ( , 212400) 摘要: 为研究草莓相关拮抗细菌的多样性, 以期筛选对草莓土传病害有良好防效的生防细菌 本研究 土传病害重发田块的草莓健株根围分离得504 株草莓根围细菌用平板对峙培养 法, 以草莓黄萎病菌草莓疫霉和草莓枯萎病菌作指示病原真菌, 筛选得24 株有显著拮抗活性 的细菌通过测定拮抗细菌的胞外酶活性( 纤维素酶, 蛋白酶, 几丁质酶) 次生代谢物( 噬铁 素) 活性, 初步确定拮抗细菌的拮抗机理通过细菌16 rDNA 限制性酶切图谱分析(ARDRA) 和16 rRNA 基因序列测定比对结果, 阐明拮抗细菌种群结构同时把分离得的拮抗细菌用于 防治草莓黄萎病试验研究结果表明, 24 个拮抗细菌的ARDRA 图谱共有12 种; 结合菌株16 rDNA 序列测定结果得出, 12 个ARDRA 类群分属于6 个细菌属( 芽孢杆菌假单胞菌伯克氏 菌土壤杆菌不动杆菌和食酸菌属) 其中芽孢杆菌假单胞菌和伯克氏菌为优势种群田间 防治结果表明, 菌株 B13 对草莓黄萎病的田间防效达935%, 菌株 B336 和 B479 的防效达 100% 关 键 词: 草莓; 土传病害; 拮抗细菌; 多样性; 生物防治 中图分类号: 436639; 476 文献 识码: A 文章编号: 2009) 增刊005206 Diversity Analysis of Antagonistic Bacteria from Strawberryrhizosphere and Their Application YANG Jinghui, ZHU Guimei, CHEN Hongzhou, PAN Yilou, WU Qingyan ( Zhenjiang Institute of Agricultural cience, Jurong, Jiangsu 212400, China) Abstract: To understandthe diversity, taxonomy and antagonistic potential of strawberryrhizosphere bac teria, and to discover new bacteria for biocontrol of strawberry soilbore pathogens, we obtained 504 bacte rial isolates from the rhizosphere of healthy strawberry in field having severity soilbore diseases. Through detecting cellulolytic, proteolytic, chitinolytic and metabolic ( siderophore) activities, and their in vitro antagonism against strawberry soilbore pathogenic fungi including Verticillium dahliae Kelb. , Phytoph thoraf ragariae Hickman and Fusarium oxysporum chl. f. sp. f ragariae Wiinks et Willams, we screened 24 candidates for controlling the above diseases. The diversity of 24 antagonistic bacteria was grouped with molecular fingerprints by using amplified ribosomal DNA restriction analysis ( ARDRA) methods. All : 20