姜黄素对HL60细胞体内体外抗癌作用的实验研究.pdfVIP

姜黄素对HL一60细胞体内、体外抗癌作用的实验研究 院血液科，福建厦门361004；3．福建医科大学药理研究所，福建福州350001) 摘要：目的研究姜黄素对急性粒细胞白血病HL-60细胞体内、体外抗癌作用。方法应用台盼蓝拒染法、MqT法及电镜观察 化等方法检测姜黄素对HL-60细胞裸鼠移植瘤的体内抗癌效应。结果①姜黄素对I-IL60细胞的增殖具有明显的抑制作用， 殖，并诱导其凋亡；可能通过下调Bcl．2蛋白表达起作用。 关键词：姜黄素；HL-60；增殖；凋亡 中图分类号：R965 文献标识码：A 文章编号：1001—2494(2005)05—0347—03 onanticancerofcurcumininHL-60cells访vitroandinvivo Study activities ZHANG Institute Pe哥，CHENYuan．zhongH，WUYon91，HUANGHui—fan91，XUJian．hua3(1．Fujianof Medical Re— Hospital，FujianUniversity，Fuzhou350001，China；2．XiamenZhongshanHospital，Xiamen searchInstitute Medical ofF@an University，Fuzhou350001，China) To theanticanceractivitiesofcureumininHL-60cellsinvitroandinvivo．METHOI)sTheeffectof ABSTRACT：OBJECrPm study curcuminonthe and ofHL-60cellsinvitrowasstudied blue and proliferationapoptosis bytrypan dye curcuminon ofHL-60cellswasevaluatedtumorinhibition DNA anticancereffectof rate，histol— fragmentationanalysis．The xenograftgrowth by and inhibited ofHL-60 inatime and ①Curcumin the cells机vitro ogy，TUNELimmunocytochemistry．RESULTSproliferation dependent dose manner．CurcumininducedHL-60cells thatwasconfirmedDNA and in— dependent apoptosis by fragmentation hibited ofHL60cellswiththeinhibitionrateof54．35％．Thereweremore cellsineracumintreatmentthan xenograftgrowth apoptie group thatinth