杂种鸡与亲本之间差异表达基因及其与杂种优势的关系.pdfVIP

业生物技术学报 Journal of Agricultural Biotechnology 2004,12 (5): 548~551 ·研究论文· 杂种鸡与亲本之间差异表达基因及其与杂种优势的关系* 孙东晓 王 栋 张 沅** 俞 英 徐桂云 李俊英 （中国农业大学动物科技学院，北京 100094 ） 摘要：利用mRNA 差异显示技术，对白洛克肉鸡、中国丝羽乌骨鸡及其正反交组合8 周龄肝脏组织的基因表达进行了分 析。发现了3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因，进一步克隆、测序和比对分析初步表明：杂种特异表达 的cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和2 个未知功能的新基因；杂种表达增强的cDNA 片断为鸡蛋白酶体亚基 基因。功能基因和调控基因在杂种鸡的特异表达和增强表达可能与部分屠体性状杂种优势形成有关。 关键词：鸡；mRNA 差异显示；杂种特异表达；杂种增强表达；杂种优势 Differentially Expressed Genes Between Chicken Hybrids and Their Parents and Their Relationship with Heterosis SUN Dong-Xiao WANG Dong ZHANG Yuan** YU Ying XU Gui-Yun LI Jun-Ying (College of Animal Science and Technology, China Agricultural University, Beijing 100094, China) mRNA differential display was used to analyze the difference of gene expression between cross combinations, EC and CE, and their parental inbreds, White Leghorn (EE) and Silkies (CC) of eight weeks old. Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit gene. The results implied that functional genes and regulatory genes which specified expressed or over-expressed in chiken hybrids were related to heterosis of some carcass traits. chicken; mRNA differential display; specified expressed gene in hybrid; over-expressed gene in hybrid; heterosis 杂种优势遗传机理研究一直是20 世纪生命科 度探讨杂种优势形成的