转录间隔区_ITS_在寄生虫分子生物学分类中的应用及其进展_牛庆丽.pdfVIP

2008, 16(4): 4147 Chinese Journal of Veterinary Parasitology 转录间隔区( ITS)在寄生虫分子生物 学分类中的应用及其进展 牛庆丽, 罗建勋, 殷 宏 (/ /, 730046) : 在寄生虫分子分类学研究中, 利用核糖体 NA 对寄生虫进行分类学, 以及虫种进化关系学的研究, 已经成 为国 上普遍应用的方法但由于它具有高度保守性的特点,所以很难在同种异株的区分上有所作为内转录间 隔区 ITS是位于寄生虫的遗传物质特别是核糖体 NA ( r NA )上 18S和 28S基因之间的区域片段, 由于它在生物 种和亚种间变异较大, 从而可以用来进行动物原虫种间以及株间的分类鉴定因此, 在分子分类学领域, 越来越多 的采用核 r NA的内转录间隔区( ITS)作为分子标记本文介绍 ITS作为分子标记在寄生虫分子分类中的作用及 其进展 : r NAITS; 寄生虫; 分子标记; 应用 : S852. 7 Q523 : A : 2008 ADVANCESAND APPLICATION OF RDNAITS IN THE MOLECULAR TAXONOMY OF PARASITE NIU Qingl,i LUO Jianxun, YIN Hong (S tate K ey Laboratory of Veter inary E tiolog ical B iology /K ey Laboratory of A nmi al P arasito logy of Gansu Pror ince, L anzhou Veter inary R esearch nstitute, CAAS, Lanzhou 730046, China) Abstract: It is ageneralmethod thatusing ribosom eRNA gene to identify the parasites and study the species evolution relation in molecular taxonomy, However, rRNA is very conservative there is little change among strain w ithin one species, so it is very difficult to identify different strains of homogeneity. ITS r NA, locating between 18S and 28S, has very high variation in biologicalspecies, was and can be used to distinguish of anmi alprotozoon species. Recently, m any papers on molecular taxonomy ofparasiteswere published using ITS as amoleculemark er. The paper described the role and progression of the ITS as amoleculemark in parasitemolecule classification. ey words: rRNAITS; parasite; moleculemarker; application 20 80, NA ( inter , nal transcribed spacers, ITS), NA, ITS 18S rRNA 16S rRNA, NA ( r NA) 18S 28S : 20080