美国通过C13和DNA扩增研究土壤呼吸.pdfVIP

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Mar. 2003, p. 1614–1622 Vol. 69, No. 3 0099-2240/03/$08.00 0 DOI: 10.1128/AEM.69.3.1614–1622.2003 Copyright © 2003, American Society for Microbiology. All Rights Reserved. Respiration of 13C-Labeled Substrates Added to Soil in the Field and Subsequent 16S rRNA Gene Analysis of 13C-Labeled Soil DNA 1 1 1 2 2 3 P. Padmanabhan, † S. Padmanabhan, C. DeRito, A. Gray, D. Gannon, J. R. Snape, 1 1 1 1 C. S. Tsai, W. Park, C. Jeon, and E. L. Madsen * 1 Department of Microbiology, Cornell University, Ithaca, New York 14853 ; AstraZeneca, Toronto, Ontario, Canada2; and AstraZeneca Global SHE, Brixham, Devonshire, United Kingdom3 Received 7 August 2002/Accepted 3 December 2002 Our goal was to develop a field soil biodegradation assay using 13C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived 13C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. The new field-based assay involved the release of 13C- labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil plots, ins