猪CAPN1基因部分外显子及3′UTR区的SNPs检测.pdfVIP

HEREDITAS (Beijing) 2008 6 , 30(6): 741―746 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00741 CAPN1 3 UTR SNPs 1 1 1 1 1 1,2 杨秀芹 , 刘慧 , 郭丽娟 , 关庆芝 , 许尧 , 刘娣 1. , 150030; 2. , 150086 : 以野猪、民猪和大白猪为研究对象, 根据网上公布的序列设计了 7 对引物, 采用测序、PCR-SSCP 和 PCR-RFLP 方法对 CAPN1 基因的部分外显子和 3′UTR 区进行了单核苷酸多态性检测和基因型分析, 探讨 CAPN1 基因多态性与瘦肉率和嫩度的关系。研究发现 11 个SNPs, 其中5 个位于外显子, 4 个位于内含子, 2 个 位于3′UTR 区, 外显子中的突变有一处是错义突变, 导致了蛋白质多肽链第260 位氨基酸发生了M/V 的替代。 群体遗传学分析表明, 在所检测的各多态位点上, 野猪、民猪、大白猪3 个品种间不同基因型的分布都存在着 极显著的差异(P0.01), 而野猪和民猪之间各基因型的分布差异不显著(P0.05), 民猪和大白猪之间各基因型 的分布存在着极显著的差异(P0.01) 。结合品种特性分析表明, P4 、P6 引物和3′ UTR 区Hin f Ⅰ位点所检测的不 同基因型和瘦肉率具有一定的相关性。 : 猪; CAPN1 基因; 瘦肉率; 嫩度; PCR-SSCP; PCR-RFLP Analysis of SNPs in partial exons and 3 UTR of CAPN1 in porcine 1 1 1 1 1 1,2 YANG Xiu-Qin , LIU Hui , GUO Li-Juan , Guan Qing-Zhi , XU Yao , LIU Di 1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China; 2. Agricultural Academy of Heilongjiang Province, Harbin 150086, China Abstract: In this study, Wild boar, Min pig and Yorkshire were used to investigate the effect of CAPN1 gene on lean meat percentage and tenderness. Seven pairs of primers for exons and 3 untranslated region of CAPN1 gene were designed ac- cording to pig sequences from GenBank. Polymorphisms within exons were detected by PCR-SSCP method. SNPs in 3 untranslated region were detected by DNA sequencing, and PCR-RFLP method was then developed to screen the population. Totally, 11 SNPs, of which 5 fr