日本血吸虫GST、Sj23及GST-Sj23 RNA颗粒抗原的制备及表达研究 Generation of GST, Sj23 and GST-Sj23 recombinant RNA particle antigens of Schistosoma japonicum and their expressions in mammalian cells.pdfVIP

中国人兽共患病学报 2009，25(5) Chinese ofZoonoses 429 Journal 文章编号：1002—2694(2009)05—0429—05 23RNA 日本血吸虫GST、sj23及CST-Sj 颗粒抗原的制备及表达研究 姜 宁1’3，尹继刚1，王心蕊1，张嘉保3，陈启军1’2 摘 要：目的 制备日本血吸虫自杀性RNA颗粒抗原，并研究其在真核细胞内的表达，以期探讨新型抗原的研制方法， 为制备抗血吸虫疫苗奠定基础。方法 对制备出的假病毒颗粒进行真核细胞感染实验，以间接免疫荧光法验证上述抗原的真核表达情况。结果 三种RNA颗粒对 BHK21细胞均具有很好的感染性，所编码的蛋白质在BHK21细胞内鉴定高效表达。 关键词：日本血吸虫；RNA颗粒抗原；真核表达 中图分类号：R383．2文献标识码：A Generationof and recombinantRNA GST，sj23GST-Sj23 particleantigens ofSchistosoma andtheir inmammaliancells japonicumexpressions Xin-rui，ZHANG JIANGNing，YINJi—gang，WANG Jia—bao，CHENQi-jun Education，Jilin 130062，China) (KeyLaboratoryofZoonosis，Ministryof University，Changchun ABSTRACT：TotherecombinantsuicideRNA—based ofSchistosomaandtheir in generate antigens japonicumexpressions two and were mammalian schistosomal sj23)anda GST-Sj23clonedinto cells，genesencoding keyantigens(GST hybridgene Semlikiforestvirus(SFV)vaccinevectorandtherecombinantsuicidalRNAvirus theschistosomaImRNA particlescontaining were BHK2l cellswith theviral withmRNA mRNA structural ofeither packagedbytransforming encoding proteins．together orthe wereharvestedandtestedfortheir tOdeliverthe mRNA GST，sj23hybridproteins．RNAparticles ability antigencoding intomammaliancells．Immuno-fluorescencewasusedtOcon