酶抑制法检测蔬菜农药残留的效果评价.pdfVIP

中国蔬菜　2013（4）：76-81 CHINA VEGETABLES 酶抑制法检测蔬菜农药残留的效果评价 叶雪珠　王　强　赵首萍　章　虎　王祥云　赵燕申 （浙江省农业科学院农产品质量标准研究所，农业部农药残留检测重点实验室，浙江杭州 310021） 摘　要：采用色谱定量检测验证方法，对目前常用的酶抑制法速测蔬菜中农药残留的效果进行 了评 价。结果表明：酶抑制法检测不同农药灵敏度差异较大，其中以克百威的灵敏度最好，最低可达 0.003 mg·kg-1 ，其次为灭多威和甲萘威。但对于目前蔬菜中常检出的毒死蜱和三唑磷等灵敏度不高。速测因最 低检测限高和基质干扰的影响，部分结果存在假阳性和假阴性风险，蔬菜产品假阳性率为 7.1%～26.2%， 而以毒死蜱为试验农药的蔬菜样品检测假阴性率较高。因此，酶抑制法速测在蔬菜高毒快速控制上发挥了 一定作用，但作为执法检测需进一步研究前处理提取方法和提高 酶试剂质量。 关键词：酶抑制法；检测；农药残留；效果 中图分类号：S63　　文献标识码：A　　 文章编号： 1000-6346（2013）04-0076-06 Effect Evaluation on Rapid Enzyme Inhibiting Method to Detect Pesticide Residues in Vegetable YE Xue-zhu，WANG Qiang，ZHAO Shou-ping，ZHANG Hu，WANG Xiang-yun，ZHAO Yan-shen Institute of Quality and Standard for Agro-Products Zhejiang Academy of Agriculture Sciences MOA Key Lab for （ ， ， Pesticide Residue Detection ，Hangzhou 310021 ，Zhejiang，China ） Abstract ：This paper evaluated the effect of daily used enzyme inhibition method to rapid detect pesticide residues in vegetables，by chromatography quantitative detection．The results showed that there were bigger differences in sensitivities of different pesticides using this method，among them sen- sitivity of carbofuran was the best，and the lowest can reach 0.003 mg·kg-1 ，followed by methomyl and carbaryl．But the sensitivities of chlorpyrifos and triazophos，which were often detected in present veg- etables，were not high．The rapid detection had false-positive and false-negative problems．The false- positive rates of vegetable products were 7.1%-26.2%，while the false-negative rates to test the pesticide chlorpyrifos for vegetables sample were generally high．Therefore，the method of enzyme inhibition has played certain role in rapid controlling high toxic vegetables，but as law enforcement detection，further research is essential to improve the enzyme reagent quality and to study the pretreatment method. Key words ：Enz