低O_2高CO_2性肺动脉高压时大鼠肺组织硫化氢体系的变化及川芎嗪的干预.pdfVIP

·524· [文章编号】1000-4718 2010 03—0524一05 低02高C02性肺动脉高压时大鼠肺组织 硫化氢体系的变化及川芎嗪的干预 贾旭广1， 石 璐1， 林丽娜2， 汪 洋1， 许益笑1， 王万铁1△ 1温州医学院病理生理教研室，浙江温州325035；2温州医学院附属第一医院麻醉科，浙江温州325000 [摘要】 目的：研究低O：高CO：性肺动脉高压时大鼠肺组织内硫化氢体系的变化及川芎嗪对其的干预作 +川芎嗪组 HH+LTZ组 。豁测其血流动力学变化，光镜观察肺细小动脉管壁面积／管总面积比值 WA／TA ，检 测右心室／左心室+室间隔 RV／LV+SP 比值，原位缺口末端标记法 TUNEL法 观测肺细小动脉凋亡情况，并 计算凋亡指数。敏感硫电极法测定血浆H：S含量及肺组织匀浆胱硫醚一．y一裂解酶 CSE 活性变化。RT—PCR法 CSE基因表达，增加CSE的活性，提高H：s水平，从而降低低O：高CO：性肺动脉高压。 [关键词]川芎嗪；肺动脉高压；细胞凋亡；胱硫醚一一裂解酶；硫化氢 [中图分类号]R363 [文献标识码]A in rats Effectof on sulfide tissueof ligustrazinehydrogensystemlung with induced pulmonaryhypertensionbyhypoxichypercapnia JIA Wan— Xu—guan91，SHILul，LINLi—na2，WANGYan91，XUYi—xia01，WANG tiel 1Department Medical 325035，China；2DepartmentofAnaesthesia，The ofPnthophysiology，WenzhouCollege，Wenzhou First WenzhouMedical AffiliatedHospimlof 325000，China．E—mail：wzwwt@tom．corn College，Wenzhou theeffectof on in [ABSTRACT]AIM：Toinvestigate ligustrazinehydrogensulfide H2S systempulmonaryhy- induced in ratswere dividedinto pertensionbyhypoxic rata．METHODS：ThirtySprague—Dawleyrandomly hypercapnia 3groups：controlgroup C ，hypoxichypercapniagroup HH ，andhypoxiehypercapnia+ligustrazinegroup HH+L ． ofvesselwall8teaandtotalareaof wereob- The of wasmeasured．Theratio arteriae changehemodynamics pulmonalis of wastestedwith dUTPnickend servedunder arteriae TdT—mediated lightmicroscope．Theapoptosis pulmonalis labeling indexwas of the calculated．Plasmalevel sulfideand of sulfide TUNEL ，andapeptosis hydrogen activityhydrogengener- ofrat tissuewereevaluated in sensitivemodifiedsulfideelectrode homogenateslung by method．Cystathi— atingenzymes in tissues ofmean wasdeterminedRT—PCR．RESULTS：Thelevel onine一-y—lyase CSE mRNAlung by pulmonary ofvesselwallarea／totalarea were arterial ratio andthe ventricle／left pressure。the fight ventricle+septumsignificandy thanthoseinC thevalue thanthatinHH inHH was lowerinHH+LTZ higher group group，and obviously group group meancarotidarterial of3 hadno in· allP O．01 ．The pressure