斜带石斑鱼(Epinephelus coioides)两种生长激素受体的克隆及其特性与组织分布研究.pdfVIP

Molecular cloning, characterization and distribution of two types of growth hormone receptor in orange-spotted grouper (Epinephelus coioides) 1# 1# 1 1 1, 2* Yun Li , Xiaochun Liu , Yong Zhang , Pei Zhu , Haoran Lin 1. State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Sun Yat-Sen University, Guangzhou, China (510275) 2. College of Ocean, Hainan University, Haikou, China (570228) E-mail ：ls32@ # These two authors contributed equally to this work Abstract The cDNAs encoding two distinct growth hormone receptors were firstly cloned and sequenced from the liver of orange-spotted grouper (Epinephelus coioides). The cDNA of grouper GHR 1 consisted of 2673-bp and encoded 658 amino acids and that of grouper GHR2 consisted of 2989-bp and encoded 577 amino acids. The two cDNAs had 78.6% identity in nucleotide sequence and 37.8% identity in deduced amino acid sequence. Northern blot analyses demonstrated a single GHR 1 transcript of approximately 4.3Kb in liver and a single GHR2 transcript of approximately 3.9Kb in the liver and muscle. In the Real-time PCR assay, grouper GHR 1 and GHR2 were expressed in all tissues tested, and the expression of GHR2 was significantly higher than GHR1 in telencephalon, cerebellum, pituitary, heart and white muscle, whereas the expression of GHR1 was significantly higher in liver. The grouper GHR1 had high homology to somatolactin receptor (SLR) and