X射线照射诱导小鼠junB基因的表达.pdf

2004 8 24 4 Chin J Radiol Med Prot, August 2004,Vol 24,No.4 319 X junB 万虹 石原弘 ! X junB 3 y X X , RNA,Northern ,junB mRNA 3 y X , junB ,C3HHe 30 min , BALBc 60min ;X , junB , 30~ 60 min , 240 min junB, , ! junB ; ; Northern ; * * Expression of junB gene inducedby Xrays in mice WAN Hong , ISHIHARA Haroshi. Bejiing Neurosur gical Institute, Beijing 100050, China Abstract! Objective To explore junB gene expression in spleen cells after whole body irradiation and in cultured spleen cells as well as leukemia cells irradiated with different doses of Xrays. Methods Spleen cells were pooled withMEMmedium after 3 y Xray whole body exposure, and normally cultured spleen cells and leukemia cells were irradiated with different doses of Xrays. Total RNA was extracted at intervals, Northern blot hybridization was used to present the expression of junB mRNA, and the RNA ratio of junBactincontrol was calculated to show the level of junB mRNA. Results After micewere exposed wholebodily to 3 y X rays, appearance of junB expression in spleen cells was remarkable and rapid, reaching its peak at 30 min for C3HHe mice, 60 min for BALBc mice. For normally cultured spleen cells and leukemia cells followed by dif ferent dose irradiation, junB mRNA increased immediately after irradiation, reaching its peak at 30 min or 60 min, returned gradually to the normal level within 240 min. Conclusion junBgene is sensitive to radiation, for it is induced immediately after irradiation. junB gene may play an important role in process of message transduc tion. Key ords! junB; Whole body irradiation; Northern blot hybridization; Signal transduction , , ,,RNA, 5 , , ( 2) : 5