高效液相色谱_串联质谱法测定减肥保健食品中酚酞.pdfVIP

分析检测 高效液相色谱-串联质谱法测定减肥保健食品中酚酞 1,2 3 1 4 3 3 3 马微 ，陈冬东 ，王海波 ，付丽 ，代汉慧 ，王秀君 ，唐英章 1.东宁出入境检验检疫局 (东宁 157200)；2.中国农业大学 (北京 100083)；3.中国检验检疫科学研究院 (北京 100123)；4.郑州 牧业工程高等学校 (郑州 450011) 摘 要 建立测定减肥保健食品中非法添加药物酚酞的高效液相色谱-串联质谱分析方法。该方法对不同 类型的减肥保健食品经超声波提取15 min，以Waters Atlantis T3柱(150 mm×2.1 mm, 3 μm)分离后，进行 HPLC/MS/MS多反应监测模式下的定性及定量分析。酚酞在(0.5~100) μg/L的范围内相关系数为0.999 4。 回收率71.3%~92.6%；日内精密度均小于8%，日间精密度均小于10%。在检测的20种样品中，检出含有酚 酞的样品9个，检出率为45%。该方法分析速度快，灵敏度高，重现性好，可用于不同减肥保健食品中非 法添加酚酞的快速检测。 关键词 高效液相色谱-串联质谱；酚酞；质谱裂解途径；基质效应 Determination of Phenolphthalein Added in the Weight-loss Functional Foods by Ultrasonication-high Performance Liquid Chromatography Tandem Mass Spectrometry 1 2 3 1 4 3 3 Ma Wei ’ ，Chen Dong-dong ，Wang Hai-bo ，Fu Li ，Dai Han-hui ，Wang Xiu-jun ， 3 Tang Ying-zhang 1.Dongning Entry-Exist Inspection And Quarantine Bureau (Dongning 157200)；2.China Agriculture University, (Beijing 100083) ；3.National Academy of inspection And Quarantine (Beijing 100123)；4.Zhengzhou College of Animal Husbandry Engineering (Zhengzhou 450011)) Abstract To develop a comprehensive analytical method for the determination of phenolphthalein in the weight- loss functional foods by high performance liquid chromatography tandem mass spectrometry. Various weight- loss functional foods were extracted under ultrasonication in 15 min. Qualitative and quantitative analysis was carried out for the 20 samples under the MRM mode after the chromatographic separation on waters atlantis T3 (150 mm ×2.1 mm, 3 μm) column. Phenolphthalein was linear in the range (0.5~100) μg/L and the linear correlation coeffi cient reached 0.999 4. The mean