犬去细胞喉支架两种体外保存方法的对照研究.pdfVIP

ClinMed ·221· J |缶床军医杂志2015年3月第43卷第3期 0mc，V01．43，No．3，March，2015 ·论著(应用基础研究)- 犬去细胞喉支架两种体外保存方法的对照研究 安 飞，马瑞娜，罗家胜，崔鹏程 (第四军医大学唐都医院 耳鼻咽喉头颈外科，陕西 西安710038) 摘要：目的比较两种体外保存方法对犬去细胞喉支架结构、软骨活性、生物力学的影响。方法将33只中华田园犬，全 保存6周时外形有明显改变，软骨细胞失活，压凹硬度试验硬度改变明显。在保存2、4周时A、B两组甲状软骨厚度、甲状 于RPMI一1640培养基，HcA器官保存液最长保存时间为4周。 关键词：喉；支架；器官保存；喉软骨；犬 中图分类号：R767文献标志码：A A oftwokindsofin、ritro methodsondeceUularizedscaffoldin An comparatiVestIldy preservation laryngeal dog Fei， Ma of andHead—neck Ruina，Luo Jiasheng，CuiPengcheng(Department0torhinolaryn90109ySurgery，Tangdu Medical Shanxi Military University，Xi’an710038，China) vitro scaf玷ldin To twokindsofin methodsondecellularized Methods Abstract：Objective presen7ation laryngeal dog． compare Chineseadult wereaUmadedecellularizedsca怕ldsand dividedintothree A Thirty—three dogs laryngeal randomly gmups．Group inRPMI一1640 nredinHCA C (n=12)was B(n=12)was organ preserved medium；Group prese preservationsolution；Gmup wasblankcontrol examinationswere (n=9) gmup．Comparative performedbymacmscopicView，histological linandeosin two—colornuorescentandbiomechanical thethree staining)，canilagedetection(FDA—PI dye) propeniesamong Results A B after and6 Cwasharvested and werehaⅣested after Gmup 1，2，4 weeks，and groups． Group