正交试验优选黄芩苷的新型提取方法_丁延虹.pdfVIP

1 Š 2 3 2 2# 丁延虹 , 丁小全 , 贾 忠 , 杨建瑜 , 贺 殿 ( 1 1 兰州市第 一人民医院, 兰州市 730050;2 1 兰州大学药学 院药物化学研究所, 兰州市 730000;3 1 兰州市肺科医院药 科, 兰州市 730046) R28412; R943 A 100 1- 0408(2009) 09- 0658- 04 目的:优选黄芩苷的新型提取方法方法: 采用液- 液连续萃取装置提取黄芩苷以总黄酮含量为筛选指标, 以溶 循环周 3 期数提取温度蒸馏时间为考察因素, 用L9 (4 ) 正交试验优选最佳提取工艺比较新型提取方法与水提酸沉法在净收率总黄酮含 量黄芩苷含量和提取物颜色方面的差异结果: 黄芩苷新型提取方法最佳工艺为溶 循环215 个周期提取温度50 e 蒸馏时间 6 h新型提取方法在总黄酮含量黄芩苷含量和提取物颜色方面均显著优于水提酸沉法结论:新方法适用于黄芩苷的提取 连续循环提取法;黄芩苷;正交试验 Optimization of New Extraction Method for Baicalin by Orthogonal Experiment ING Yan_hon (Lanzhou First Munici al Peo lecs Hos ital,Lanzhou 730050, China) g p p p ING Xiao_ uan, YANG Jian_ u, HE ian( Institute of Medicinal Chemistr , School of Pharmac , q y y y Lanzhou University, Lanzhou 730000, China) JIA Zhon ( e t1 of Pharmac , Lanzhou Hos ital of Pulmonar iseases, Lanzhou 730046, China) g p y p y ABSTRACT OBJECT IVE: T o explore a new extraction method of baicalin1 METHO S: A new continuous circular ex- 3 traction equipment was adopted to extract baicalin1 L9( 4 ) orthogonal experiment was conducted to optimize the extraction process taking the content of total flavonoids as index with the number of period of batching cycle of solvent, extraction tem- perature and distillation time as factors1 T he new continuous circular extraction was compared with the aqueous extraction and acidic deposition method in terms of the yield of extractum, content of total flavonoids and baicalin content and the color of the extract1 RESU LTS: T he best technical conditions for the new extraction m