芦苇叶类黄酮高效液相色谱分析.pdfVIP

※分析检测 食品科学 2011, Vol. 32, No. 10 241 芦苇叶类黄酮高效液相色谱分析 孙丽芳，刘邻渭* ，吕俊丽，李 旋 (西北农林科技大学食品科学与工程学院，陕西杨凌 7 12 100) 摘 要：目的：分析芦苇叶类黄酮的组分。方法：制备芦苇叶类黄酮提取浓缩过程中形成的析出物和浓缩液再经 大孔树脂纯化后产生的初纯物，优化两种样品中类黄酮苷转化为苷元的水解条件，采用高效液相色谱(HPLC)法测 定两种样品和其水解样品类黄酮的组分。结果：析出物和初纯物的总黄酮含量分别为57 . 1% 和18.5% ，酸浓度 1.4mol/L 、温度80 ～90 ℃条件下水解4h 可获得相对最好的水解效果；HPLC 测定表明，析出物含32 种组分，其 中7 种得到确定，初纯物含22 种组分，其中5 种得到确定；水解析出物含28 种组分，其中7 种得到确定，水解 初纯物含2 5 种组分，其中7 种得到确定。结论：芦苇叶含有芦丁、野黄芩苷、橙皮苷、木犀草素、槲皮素、 芹菜素、山奈酚、异鼠李素(或橙皮素) 、异甘草素和黄芩素，其中芹菜素含量最高，还有许多未确定的类黄酮。 关键词：芦苇叶；类黄酮；水解；高效液相色谱(H P L C) HPLC Analysis of Flavonoids from Reed Leaves . . SUN Li-fang ，LIU Lin-wei* ，LU Jun-li ，LI Xuan (College of Food Science and Technology, Northwest A F University, Yangling 7 12 100, China) Abstract：Obj ective: To analyze the compositions of flavonoids from reed leaves. Methods: The crude extract was prepared during the conden sation of reed leaf extract. The primarily purified flavonoids were prepared by macro-porou s resin . The hydroly sis condition s of flavonoids were optimized. Two samples were hydrolyzed to obtain two hydroly sis products. The composition s of flavonoids were determined by HPLC. Results: The contents of total flavonoids in crude extract and primary purified flavonoids samples were 57.1% and 18.5%, respectively . The optimal hydrolysis conditions for reed leaf flavonoids were hydroly sis temperature of 80 －90 ℃, HCl concentration of 1.4 mol/L and hydrolysis time of 4 h . Totally 33 components were separated by HPLC from the crude extract and 8 components were identified based on their retention time. A total of 22 component s