利用改进的酚氯仿法从猪毛囊中提取基因组DNA.pdfVIP

HEREDITAS (Beijing) 2010 7 , 32(7): 752―756 ISSN 0253-9772 技术与方法 DOI: 10.3724/SP.J.1005.2010.00752 利用改进的酚-氯仿法从猪毛囊中提取基因组DNA 1, 2 1 1 2 1 王继英 , 俞英 , 冯利霞 , 王怀中 , 张勤 1. , 100193; 2. , 250100 为提高从猪毛囊组织中提取基因组 DNA 的效率, 文章在借鉴从其他组织提取基因组 DNA 方法的基础上, 对经典的酚-氯仿法的反应体系和步骤进行了改进。利用改进的酚－氯仿抽提法, 从猪的毛囊组织中快速、高效 地提取了高质量基因组DNA 。利用该方法从 1~6 根猪毛囊中提取的基因组 DNA 可满足基于PCR 技术的相关 分子生物学实验需要。 猪毛囊组织; 基因组DNA; 酚-氯仿法 Genomic DNA extraction from hair sacs of pigs using modified phenol-chloroform method 1, 2 1 1 2 1 WANG Ji-Ying , YU Ying , FENG Li-Xia , WANG Huai-Zhong , ZHANG Qin 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2. Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, Jinan 250100, China Abstract: In referring to various methods for genomic DNA extraction from different tissues, we modified the classical phenol–chloroform procedure and reaction system for use in genomic DNA extraction from pig hair sacs. With the modified the phenol–chloroform method we successfully obtained high quality genomic DNA from pig hair sacs. Genomic DNA can be extracted from sacs of one to six pig hairs with satisfied quantity and quality for the need of PCR-based molecular ex- periment. Keywords: pig hair sac; genomic DNA extraction; phenol–chloroform method , DNA DNA [2~6], , , , DNA , DNA : , , DNA ; ;