非标记纳米金光谱法检测雌激素.docVIP

非标记纳米金光谱法检测雌激素 余玲1,2，，，，* 1商丘师范学院化学化工学院，河南 商丘，476000 2中南大学化学化工学院，湖南 长沙，410012 3郑州大学化学与分子工程学院，河南 郑州，450001 Email: xumaotian@sqnc.edu.cn 本文利用可见光度法研究了纳米金粒子与雌酮的相互作用。首先利用经典的柠檬酸钠还原氯金酸法制备得到了均一稳定的纳米金粒子，平均粒径为35 nm。在pH=5.72的BR缓冲体系中，纳米金与雌酮发生相互作用，吸收光谱明显红移。吸光度与雌酮浓度的对数值在0.01～10 ×10-6 mol?L-1范围内呈线性关系，检测限为4.7×10-9 mol?L-1。本研究建立了一种快速、方便检测雌激素的新方法，为奶粉、食品中雌激素的检测提供了一条新的途径。 Fig. 1 Structure of estrone Fig. 2 Visible absorption spectra of gold nanoparticles in the presence of difference concentrations of estrone 关键词：可见光度法 参考文献 [1] Frens G. Nat. Phys. Sci. 1973, 241: 20-22 [2] Wang Z, Ma L. Coord. Chem. Rev. 2009, 253(11-12): 1607-1618 [3] Kushnir MM, Rockwood AL, Yue B, Meikle AW. Methods Mol. Biol. 2010, 603: 219-28 Spectroscopy detection of estrogen using non-labeled gold nanoparticles Ling Yu1,2, Yanzheng Zhang 3, Yadan Wang3, Yintang Zhang1, Maotian Xu1,3* 1College of Chemistry and Chemical Engineering, Shangqiu Normal University, Shangqiu 476000 2College of Chemistry and Chemical Engineering, Central South University, Changsha 410012 3College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001 The interaction of gold nanoparticles (AuNPs) with estrogen was studied by visible spectroscopy. Firstly, AuNPs were prepared through reduction of chloroauric acid by sodium citrate. The resultant nanoparticles were with good dispersity, and the mean particle diameter was about 35 nm. The absorption spectra had obvious red shift when AuNPs encountered estrone in Britton-Robinson pH 5.72 buffer. A good linear relation between absorption and logarithm concentration of estrone was found in the range of 0.01～10×10-6mol·L-1 and with detection limit of 4.67×10-9mol·L-1. This assay was fast, low-cost and readily used to detect estrogen in milk powder and food.