超声法提取土茯苓中总黄酮的工艺研究.pdf

·60· Chinese Journal of Information on TCM Jun.2011 Vol.18 No.6 超声法提取土茯苓中总黄酮的工艺研究 1 2 2 1 1 1 姚冬梅 ,刘开蕾 ,周长青 ,王锐 ,吕邵娃 ,李永吉 (1.黑龙江中医药大学,黑龙江 哈尔滨 150040；2.牡丹江医学院红旗医院二发电分院,黑龙江 牡丹江 157000) 摘要：目的 研究超声法提取土茯苓中总黄酮的最佳工艺。方法 设计正交试验,采用超声法提取,以总黄酮提 取率为指标,对土茯苓中总黄酮的提取工艺进行研究。结果 超声法提取土茯苓总黄酮的最佳工艺为：乙醇浓度60%, 乙醇用量15倍,提取时间45 min,提取温度60 ℃。结论 该工艺方法简单,耗时短,提取率高。 关键词：土茯苓；总黄酮；超声提取；正交试验 DOI：10.3969/j.issn.1005-5304.2011.06.026 中图分类号：R283.5 文献标识码：A 文章编号：1005-5304(2011)06-0060-03 1 Technology Study on Ultrasonic Extraction of Total Flavonoids in Smilax glabra YAO Dong-mei , 2 2 1 1 1 LIU Kai-lei , ZHOU Chang-qing , WANG Rui , LV Shao-wa , LI Yong-ji (1.Heilongjiang University of Chinese Medicine, Harbin 150040, China ；2.Red Branch Hospital, the Second Generation, Mudanjiang Medical College, Mudanjiang 157000, China) Abstract ：Objective To explore the best extraction technique of total flavonoids in Smilax glabra with ultrasonic-assisted technique. Methods The orthogonal design was used in the ultrasonic-assisted technique with the extraction rate of total flavonoids as the index. Results The optimum conditions of the extraction were as follows：the amount of 60% ethanol was 15 times, extracting temperature was 60 ℃ and extracted for 45 min. Conclusion This process was featured by higher extraction rate and shorter extraction time. Key words ：Smilax glabra；total flavonoids；ultrasonic extraction；orthogonal design 土茯苓为百合科植物光叶菝葜 Smilax glabra Roxh.的干 乙醇,室温下浸泡 12 h 后超声提取,残渣用相同浓度和体积的 燥根茎,其味甘、淡,性平,归肝、胃经,是清热解毒常用中药, 乙醇再超声提取 1 次,合并 2 次提取