精制逍遥散对抑郁症大鼠行为学及其海马区P450scc蛋白表达的影响.pdfVIP

精制逍遥散对抑郁症大鼠行为学及海马区 P450scc蛋白表达的影响 郭晓玲1张轶芳1刘玥芸1赵宏波1,2 岳广欣3陈家旭1 1北京中医药大学，北京1000292北京市石景山区中医医院，北京100041 3中国中医科学院中医基础理论研究所，北京100700 摘要：目的：探讨精制逍遥散治疗抑郁症的作用机制。方法：将40只健康SD雄性大鼠随机分为正常组、模 型组、逍遥散组和氟西汀组，后3组进行大鼠抑郁症造模。逍遥散组、氟西汀组于实验的第1天开始分别灌服精制 逍遥散、氟西汀，每日1次。21d后观察大鼠行为学以及海马区P450scc蛋白表达的变化。结果：抑郁症模型大鼠体 质量增长缓慢，糖水消耗率降低，摄食潜伏期延长，5min内移动的速度减慢，5min内移动总距离、进入中央区停 留时间减少；海马CA3和DG区P450scc蛋白表达明显减弱 （P＜0.05,P＜0.01）。逍遥散组及氟西汀组体质量、糖水 消耗率、摄食潜伏期、5min内移动速度及总距离和中央区停留时间与模型组比较有统计学意义，CA1区相对增加， 并能有效逆转抑郁症大鼠海马CA3和DG区P450scc蛋白表达。结论：大鼠抑郁模型脑区存在神经活性甾体的功能紊 乱，逍遥散治疗抑郁症其机制可能是通过调节P450scc蛋白表达。进一步影响神经活性甾体的功能而实现的。 关键词：精制逍遥散；抑郁症；大鼠；行为学；海马；P450scc EffectsofrefinedXiaoyaoPowderonethologyandexpressionsofP450sccin hippocampusofthedepressionrat GUOXiao-ling1ZHANGYi-fang1LIUYue-yun1ZHAOHong-bo1，2YUEGuang-xin3CHENJia-xu1 1BeijingUniversityofChineseMedicine,Beijing100029,China 2HospitalofTCMSShijingshanDistrictofBeijing,Beijing 100041,China 3InstituteofBasicTheoryofTCM,ChinaAcademyofChineseMedicalSciences,Beijing100700,China Abstract:Objective:ToexplorethemechanismofrefinedXiaoyaoPowderfortreatmentofdepression.Methods:Forty healthySDmaleratswererandomLydividedintoanormalgroup,amodelgroup,aXiaoyaoPowdergroupandaFluoxetine group.Thedepressionratmodelwasestablishedinthelatterthreegroups.Fromthefirstdayoftheexperiment,theXiaoyao PowdergroupwasorallyadministratedtherefinedXiaoyaoPowoleronceeveryday;theratsintheFluoxetinegroupwere treatedwithFluoxetineonceeveryday.Aftertreatmentof21days,changesofethologyandexpressionofproteinP450sccin hippocampuswereobservedintherats.Results:inthedepressionmodelrats,thebodymassinereasedslowly,sucrosepreference decreasedandingestionlatencystageextended,movementspeedwithin5minreduced,andtotalmotiondistancewithin5min, stayperiodincenterzonedecreased;theexpressionofP450sccintheCA3andDGregionsofhippocampussignificantlyreduced (