氯化钙对戊巴比妥钠致蟾蜍心力衰竭地拮抗作用.docVIP

氯化钙对戊巴比妥钠致蟾蜍心力衰竭的拮抗作用 赵小康 ， 王蓓蓓， 李清 ， 胡代菊， 马珺， 赵晶晶 [摘要] 目的：观察氯化钙对戊巴比妥钠诱发心衰的作用。方法：斯氏法游离蟾蜍心脏，用含戊巴比妥钠（2mmol/L,1ml）的任氏液造成蟾蜍心衰模型（心肌收缩力降低一半时为心衰），然后加入氯化钙（90mmol/L,0.04ml）,分别记录药前及药后心肌收缩力和心率。用任氏液清洗至基本恢复正常后改用含戊巴比妥钠（2mmol/L,1ml）的任氏液和氯化钙（90mmol/L,0.04ml）的混合液灌流心脏，并记录心肌收缩力和心率变化。结果：用含戊巴比妥钠的任氏液灌流心脏使心肌收缩力降低，心率变慢。氯化钙可拮抗心肌收缩力的降低（P0.05）。结论：氯化钙可拮抗戊巴比妥钠诱发的心衰。 [关键词] 戊巴比妥钠；心力衰竭；蟾蜍；氯化钙 Effects of chloride calcium on heart failure caused by pentobarbital sodium in toads ZHAO Xiao-kang,WANG Bei-bei,LI Qing,HU Dai-ju,MA Jun,ZHAO Jing-jing [Abstract] Objective: To observe the effect of chloride calcium on heart failure in toads. Methods:The heart failure models were produced by perfusing Ringer’s solution containing pentobarbital sodium (2mmol/L,1ml) in isolated toad hearts. When the myocardiac contractile force decreased over 50 percent of the normal index, chloride calcium (90mmol/L,0.04ml) was added to antagonize it. The myocardiac contractile force and heart rate were recorded separately before and after giving drugs. After washing, the toad heart was perfused by the mixture of pentobarbital sodium (2mmol/L,1ml) and chloride calcium (90mmol/L,0.04ml). The parameters were taked down. Results: When adopting pentobarbital sodium to make a model of heart failure in toads, the myocardiac contractile force reduced and heart rate also lowered. The reduction of myocardiac contractile force can be antagonized by chloride calcium (P0.05). Conclusion:Chloride calcium can antagonize the heart failure induced by pentobarbital sodium. [Key words]: pentobarbital sodium; heart failure; toad; chloride calcium 戊巴比妥钠是镇静催眠药。在实验研究中常用来诱发心衰。如李青等用戊巴比妥钠诱发蟾蜍心衰[1]，王亚芳等用其诱发猫心衰[2]，孙文娟等用其诱发家兔心衰［4］。但引起心衰的机制尚不清楚。本实验主要观察氯化钙对戊巴比妥钠诱发的心衰的影响，探讨戊巴比妥钠引起心衰的机制。 材料与方法 1．1 药物和仪器 戊巴比妥钠(pentobarbital sodium,PB),上海化学试剂采购供应站分装厂提供；无水氯化钙（Chloride calcium），蚌埠电化试剂厂提供。二道生理记录仪，成都仪器厂（LMS-2B型）。 1．2 动物 中华大蟾蜍，雌雄兼用，10只，由蚌埠医学院实验动物科提供。 1．3 方法 斯氏法游离蟾蜍心脏，并进行心脏插管，注入任氏液1ml。用试管夹将蛙心管固定，用蛙心夹夹住心尖，接张力传感器，并与二道生理记录仪相连，记录心脏搏动曲线，仪器定标为1g=7mm作为对照