酶联免疫吸附试验在食品检测中的应用.pdfVIP

※专题论述 食品科学 2009, Vol. 30, No. 23 489 1 2 3 王守法 ，阚春月 ，许学书 (1.华东理工大学生物工程学院，上海 200237 ；2.上海海洋大学食品学院，上海 201306 ； 3.华东理工大学生物反应器工程国家重点实验室，上海 200237) 本文简要介绍了ELISA 的基本原理和类型，详述了国内外使用ELISA 方法在食品过敏源、农药残留、致 病微生物、转基因等方面检测的最新研究进展，列出了部分目前市售用于食品检测的试剂盒，并对该检测技术的 发展趋势及其在食品安全检测中的应用前景进行了展望。 酶联免疫吸附试验(ELISA) ；食品检测；免疫分析；ELISA 试剂盒 Application of ELISA Method in Food Detection 1 2 3 WANG Shou-fa ，KAN Chun-yue ，XU Xue-shu (1. College of Bioengineering, East China University of Science and Technology, Shanghai 200237, China； 2. College of Food Science and Technology, Shanghai Ocean University, Shanghai 201306, China； 3. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China) Abstract：The principle and types of enzyme linked immunosorbent assay (ELISA) were introduced, and the applications of ELISA in analysis of food investigation progress were reviewed, including the determination of anaphylactogen, pharmaceuticals residue, food toxins, pathogenic micro organisms and the compositions of genetically modified foods, etc. Some of the ELISA kits available in the market were listed. And the trends in the development of ELISA and its prospect in the food safety determination were also forward commented. Key words ：enzyme linked immunosorbent assay(ELISA)；food determination ；immunoassay ；ELISA kits 中图分类号：TS207.3 文献标识码：A 文章编号：1002-6630(2009)23-0489-04 酶免疫技术是20 世纪60 年代在免疫荧光和组织化学 ELISA(enzyme linked immunosorbent assay)是在免疫 基础上发展起来的一种新技术，最初是用酶来代替荧光