环境因子对水霉菌生物膜形成地影响袁海兰.pdfVIP

Sep. 20, 2014, 41(9): 1829−1836 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.130732 ( 201306) (Saprolegnia) (BiofilmBF) (Saprolegnia parasitica) ATCC200013 96 CCK-8 (Cell Counting Kit-8) OD450 24 h 48 h OD450 (P0.05) 20−25 °C OD450 (P0.05) pH 4−11 0.12 mmol/L CaCl2 0.03−2.00 mmol/L MgCl2 MgCl2 Cu2+ 0.5 mmol/L NaCl NaCl 0.12% (P0.05) Effects of environmental factors on biofilm formation of Saprolegnia YUAN Hai-Lan SU Jian HU Kun CAO Hai-Peng YANG Xian-Le* (State Collection Center of Aquatic Pathogen, Shanghai Ocean University, Shanghai 201306, China) Abstract: [Objective] Biofilm of Saprolegnia was developed in vitro and the effects of the environmental factors were studied. [Methods] The characteristics of biofilm formation of Saprolegnia parasitica ATCC200013 were investigated by modified microtiter-plate test, and the viability of Saprolegnia in the biofilms was detected by CCK-8. [Results] The OD450 value of Saprolegnia biofilm reached the peak after 24 h and stabilized after 48 h. OD450 value of biofilm significantly elevated with densities of initial Saprolegnia spores (P0.05). The largest biofilm quantity was at 20 °C and 25 °C, which were significantly higher than the others (P0.05). Saprolegnia can develop biofilm at initial pH between 4 and 11. When more than 0.12 mmol/L CaCl2 863 (No. 2011AA10A216) (No. (