简化基因组+转录组研究狗岩螺多样性.pdf

Molecular Ecology (2014) 23, 1863–1873 doi: 10.1111/mec.12681 Phylogenomic analyses reveal latitudinal population structure and polymorphisms in heat stress genes in the North Atlantic snail Nucella lapillus NATHANIEL D. CHU,* † STEFAN T. KALUZIAK, † GEOFFREY C. TRUSSELL † and STEVEN V. VOLLMER † *Ecology and Evolutionary Biology, Brown University, 80 Waterman St., Box G-W, Providence, RI 02912, USA, †Marine Science Center, Northeastern University, 430 Nahant Road, Nahant, MA 01908, USA Abstract North Atlantic rocky intertidal species have been shaped by repeated glaciations and strong latitudinal temperature gradients, making them an excellent system to study postglacial phylogeography and thermal tolerance. Population genetics data from northwestern Atlantic species, however, often show patterns inconsistent with the pre- diction that high dispersal should generate weaker genetic structure among popula- tions. Here, we used next-generation sequencing restriction-associated DNA tags (RAD-seq) and a transcriptome assembled from RNA-seq data to analyse the genetic structure of northwestern Atlantic populations of the low-dispersal intertidal snail Nucella lapillus. Although previous studies in this region have detected almost no genetic structure in N. lapillus, our phylogenomic approach identified a well-supported split between northern and southern clades. By comparing RAD-seq data and our tran- scriptome assembly, we identified thousands of fixed single-nucleotide polymorphisms (SNPs) between these latitudinal clades that map to protein-coding gene