硫化氢和一氧化氮对心梗大鼠的心脏保护作用及相互关系.pdfVIP

JournalofChinesePharmaceuticalSciences f ：／／www．jcps．ac．cn Cardi0pr0tectiVeeffectsofhydrogensulfideandnitricoxideandtheir interactionsduringtheprocessofmyocardialischemiainrats Chang．QingChen ，HongXin ，Yi—ChunZhu，Yi—ZhunZhu 1．DepartmentofPharmacology,School PharmacyandlnstituteofBiomedicalSciences,FudanUniversity,Shanghai20003ZChina 2．DepartmentofPhysiologyandPathophysiology,FudanUniversiyt,Shanghai200032,China Abstract：Therelationshipbetweenhydrogensulfide(H2S)andnitricoxide(NO)inmyocardialinfarctionfMI)hasnotbeen previouslyreported．Inthecurrentinvestigation．wesoughttodeterminetherolesofboht H2SandNO inMInirats．Animalswere randomlydividedinto5groupsandtreatedwi血L—NG—nitroargininemethylester(L—NAME)，sildenafil，saline，propargylglycine (PAG)andL-cysteine，respectively，for1weekpriortoperformingMIsurgeryorshamoperation．Themortalityrateswerelower insildenafilandL．cysteinetreatedratsintheMIgroup．Thenifarctareawassignificantlyreducedinsildenafi1andL-cysteine treatedrats．Moreover．plasmaH，Smeasurementsrevealedthatthelevelinthesildenafiltreatedgroupwaslowerthaninthe L．NAMEtreatedMIgroup．whichwasconsistentwihtallobserveddecreaseincystahtioninegamma．1yase(CSE)enzymeactiviyt． CSE proteinexpression1evelintheL—NAV／IE treatedM Igroupwassignificantlyhigherthna insildenafi1treatedMIgroup． eNOSproteincontentinhteL—cysteinetreatedMIgroupwaslowerthaninthePAGtreatedMIgroupandeNOSgeneexpression issignificantlydecreasedintheL．cysteinetreatedrats．W edemons仃atedthatendogenousH，SandNO arecardioprotectiveinthe ratmode1ofMI．Indeed，boththeH2S—CSEandNO_NOSsystem appeartohaveamutua1down．regulationeffectinMIprocess inourexperimentalratmode1． Keywords：Hydrogensulfide；Nitricoxide；Cystathioninegamma．1yase；Myocardialinfarction CLCnumber：R969．2 Documentcode：A ArticleID：1003—1057(2009)1—64—9 1．Introduction Itwasreported thatthemechanismsunderlying