《Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes》.pdfVIP

Cancer Biol Med 2013;10:221-226. doi: 10.7497/j.issn.2095-3941.2013.04.006 ORIGINAL ARTICLE Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes 1 2 1 1 1 1 1 1 Xin-Ying Zhang , Fu-Qiang Yin , Li Liu , Ting Gao , He-Yun Ruan , Xiao Guan , Ying-Xin Lu , Dan-Rong Li 1 2 The Cancer Hospital Affiliated to Guangxi Medical University, Nanning 530021, China; The Experiment Center of The Guangxi Medical University, Nanning 530021, China AbstrAct Objective: To study explores the effect of HLEC on the secreted proteins of epithelial ovarian cancer (EOC) cells (SKOV3-PM4) with directional highly lymphatic metastasis. Methods: Supernatants of four groups of cultured cells, namely, SKOV3 (A), SKOV3+HLEC (B), SKOV3-PM4 (C), SKOV3-PM4+HLEC (D), were collected, and their proteins were detected by antibody arrays and iTRAQ-2D-LC-MALDI- TOF/TOF/MS. Significantly differential proteins were further analyzed via bioinformatics and validated in human serums and cell media via ELISA. Results: Results of antibody arrays and mass spectrometry demonstrated that GRN and VEGFA were upregulated in group C (compared with group A), whereas IGFBP7 and SPARC were downregulated in group D (compared with group C). Comprehensive bioinformatics analysis results showed that IGFBP7 and VEGFA were closely linked to each other. Further validation with serums showed statistical significance in VEGFA and IGFBP7 levels among groups of p