Development of a multilayer microfluidic array chip to culture and replate.pdfVIP

View Online / Journal Homepage / Table of Contents for this issue COMMUNICATION /loc | Lab on a Chip Development of a multi-layer microfluidic array chip to culture and replate uniform-sized embryoid bodies without manual cell retrieval† a a a b a Edward Kang,‡ Yoon Young Choi,‡ Yesl Jun, Bong Geun Chung * and Sang-Hoon Lee* Received 7th May 2010, Accepted 14th July 2010 DOI: 10.1039/c0lc00005a We have developed a multi-layer, microfluidic array platform con- EBs.15 Concave microwells, similar to the contour of EBs, generated taining concave microwells and flat cell culture chambers to culture homogeneous EBs, resulting in EB size-mediated neuronal differen- A embryonic stem (ES) cells and regulate uniform-sized embryoid tiation. Quantitative analysis showed that the neurite numbers and 5 average lengths of larger EBs were much higher than those of smaller 0 body (EB) formation. The main advantage of this platform was that 0 0 EBs. However, this approach also required pipettes to harvest the 0 EBs cultured within the concave microwells of a bottom layer were C L automatically replated into flat cell culture chambers of a top layer, EBs and replate them onto laminin substrates. 0 C