枯草芽孢杆菌产凝乳酶发酵条件的优化.pdfVIP

156 2011, Vol. 32, No. 03 食品科学 ※生物工程 枯草芽孢杆菌产凝乳酶发酵条件的优化 丁明亮，欧阳安然，王望斐，顾正华，丁重阳* ，张 梁，石贵阳 (工业生物技术教育部重点实验室，江南大学生物工程学院，江苏无锡 2 14 122) 摘 要：为进一步提高枯草芽孢杆菌(Bacillus subtilis)液态发酵产凝乳酶(milking-clotting enzyme，MCE) 的能力，采 用单因素试验和响应曲面法对枯草芽孢杆菌液态发酵的产酶条件进行研究和优化。结果表明：最优发酵工艺为： 葡萄糖添加量16.2g/L ，在500mL 三角瓶中装53.3mL 料液，接种量为体积分数0.130% ，发酵时间120.43h ，预测 酶活力为1097.30SU/mL ，经实验验证，在该条件下发酵产凝乳酶的酶活力为( 1129.05 ±74.55)SU/mL ，与模型预 测值相符。 关键词：凝乳酶；枯草芽孢杆菌；液态发酵； 响应曲面法；优化 Optimizing the Fermentation Process for Milk-clotting Enzyme Production by Bacillus subtilis DING Ming-liang ，OUYANG An-ran ，WANG Wang-fei ，GU Zheng-hua ，DIN G Zhon g-yan g* ， ZHANG Liang ，SHI Gui-yang (Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 2 14 122, China) Abstract ：In order to improve the yield of milk-clotting enzyme (MCE), response surface methodology (RSM) was used to optimize the fermentation process for the production of milk-clotting enzyme from Bacillus subtilis under liquid fermentation. The results showed that the optimal parameters for the fermenting process were as follows: glucose 16.2 g/L, 53.3 mL medium in a 500 mL Erlenmeyer flask, inoculums size 0.130%, fermenting time 120.43 h, and the predicted MCE yield was 1097.30 SU/mL. Under the optimal conditions the MCE activity was (1129.05 ±74.55) SU/mL, indicating that the optimizing process is effective. Key words ：milk -clottin g enzym e ；B acillus subtilis ；subm erged ferm entation ；resp on se surface meth odology ； optimization 中图分类号：Q8 14.4 文献标识码：A 文章编号：1002-6630(20 11)03-0 156-05 凝乳酶是乳酪生产中的关键酶，传统上利用牛犊第