Insights into its Sweetness and Taste-modifying Activity外文翻译.pdfVIP

doi:10.1016/j.jmb.2006.03.030 J. Mol. Biol. (2006) 359, 148–158 Crystal Structure of Neoculin: Insights into its Sweetness and Taste-modifying Activity 1 1 2 3 Akiko Shimizu-Ibuka †, Yuji Morita †, Tohru Terada , Tomiko Asakura 1 4 1 1 Ken-ichiro Nakajima , So Iwata , Takumi Misaka , Hiroyuki Sorimachi Soichi Arai5 and Keiko Abe1* 1Department of Applied Although the majority of sweet compounds are of low molecular mass, Biological Chemistry several proteins are known to elicit sweet taste responses in humans. The fruit Graduate School of Agricultural of Curculigo latifolia contains a heterodimeric protein, neoculin, which has and Life Sciences, The University both sweetness and a taste-modifying activity that converts sourness to ˚ of Tokyo, 1-1-1 Yayoi, Bunkyo-ku sweetness. Here, we report the crystal structure of neoculin at 2.76 A Tokyo 113-8657, Japan resolution. This is the first well-defined tertiary structure of a taste-modifying 2 protein of this kind. The overall structure is quite similar to those of monocot Agricultural Bioinformatics mannose-binding lectins. However, crucial topological differences are Research Unit, Graduate School observed in the C-terminal regions of both subunits. In both subunits of of Agricultural and Life Sciences