翘嘴鳜miR-222的表达特征280.pdfVIP

1 The expressional characterization of miR-222 in mandarin 2 fish (Siniperca Chuatsi) 1 2 2 3 1 2 2 3 ZHU Xin , HU Yi , WANG Kai-Zhuo , WU Ping , YI Tan , CHEN Dun-Xue , ZHANG Jun-Zhi 1 4 and CHEN Tao 5 6 (1. College of Veterinary Medicine, Hunan Agriculture University, Changsha 410 128, China; 2. College of Animal 7 Science and Technology, Hunan Agricultural University, Changsha 410128, China; 3. College of Biology, Hunan 8 University, Changsha 410082, China) 9 10 Abstract: The mandarin fish (Siniperca chuatsi Basilewsky) is one of the most 11 commercially important carnivorous fish species in aquaculture. Increasing evidences 12 have suggested that microRNAs (miRNAs) have emerged as key regulators of 13 skeletal muscle growth, but little is known about miRNA expression profiles relate to 14 skeletal muscle growth in teleost. The miR-222 has been reported as the key regulator 15 during muscle differentiation. In the present study, we found that the miR-222 was 16 highly expressed in muscle-related tissues, especially in fast (white) muscle of adult 17 mandarin fish. RT-PCR analysis revealed that the miR-222 was first detected in two 18 cell stage embryos and highly expressed at the heart beating stage. We investigated 19 the expression changes of the mature miR-222 abundance following a single satiating 20 meal in mandarin fish juveniles after fasting for a week. MiR -222 expression was 21 sharply up-regulated within 1h after refeeding and might be