中药脑神康方化学成分及质量控制方法研究.pdf

S tu d y o f ch e而 ca l in gr ed ien ts an d q u a lity co n tro lm eth od s o f th e ，’N a o S h en K a n g ，， tr a d itio n a l C h in e se M e d ic in e S P eeia llty :P h amr aey o f Tr ad ition alC h in ese M ed icin e A u th o r :Z H O N G L i一h o n g Tu to r :P ro fesso r L IU X ian g 一h o n g A b str a C t o b jeetiv e :To Prelim inari ly stUdy the eh em ical in gredients an d the corresP on ding qu ality con tro lm eth od for the “N ao Sh en K an g ”困 SK )，a Trad ition al C h in ese M ed icin e ean b e u sed for rem e d y o f d iab ete s a sso e iate d w ith b ra in fu n etio n d eerea se s. M eth o d s : 、乞行ety eo lu m n eh ro m ato gr ap h y w ere u sed to in v e stig ate ch em ic a l co n stituen ts a n d stru eture id en tif icatio n o f N S K . T h e T L C an d H P L C m eth o d s w ere u sd e d to re se areh th e co n ten t o f a etiv e in gr ed ien ts an d lim ited in gre d ien ts in N S K . R e su lts : S ix e o m P ou n d s w ere iso la ted from N S K . F iv e o f w h ich id en tif ied b y sP eetraldata and eh em iealev id ences，w ere (1)B 一sito sterol、 (3) gen istein 、 (4 ) P u erari n 、 (5) gen istin 、(6) icari in . A ln on g them ，com Pound 2 w as n ot identified for the little am oun t. T h e T L C w a s set u P for id en t if icatio n o f the A strag a lo sid e ，b eta in e ，icari in ，b erb er in e ，an d P u erari n in N S K .A t th e sam e tim e ，the d eterm in atio n m e tho d o f P o ly sa cch ari d es，P u erari n ， ieari in ，b erb er in e an d a stra g a lo sid e w ere e stab lish ed . F u rht emr o re ，