寿胎丸调节转录因子T-bet%2fGATA-3比率逆转习惯性流产Th1%2fTh2失衡机制的研究.pdf

The mechanism study about shoutaiwan on adjusting transcription factors T-bet/GATA-3 ratio to reverse Th1/Th2 in RSA Speciality: Integrated Chinese and Western Medicine Gynaecology Author: WEN Ji Tutor: Professor SUN Wei Abstract Objective: To study the mRNA expressions of GATA-3 、T-bet and the level of IL-2、 IFN-γ、 IL-4 、IL-10 of blood before and after using shoutaiwan, to confirm shoutaiwan adust the ratio of T-bet/GATA-3 by upregulating expression of GATA-3 and downregulating expression of T-bet and restore Th1/Th2 cytokines balance, thus to maintain the pregnancy of habitual abortion patients. Methods: 50 RSA patients were randomly divided into two groups of A and B. Group A include 30 cases which were given shoutaiwan;group B include 20 cases which were blank control and group C include 20 healthy pregnant women. Patients in three groups were pumped peripheral venous blood two copies, one piece of blood was centrifugalized out plasma, the levels of IL-2、IFN-γ、IL-4、IL-10 of plasma were measured using ELISA method. Other piece of blood was separated PBMCs according to the density gradient centrifugation. mRNA expression of T-bet and GATA-3 of PBMCS were measured by the real-time quantitative polymerase chain reaction. Results: the level of IL-2 and INF-γ were significantly higherof patients in habitual abortion groups (group A +groupB) than those healthy pregnant women in group C, mRNA expressions of GATA-3, the level of IL-4 and IL-10 were lower to the contrary(P0.05); The mRNA expressions of T-bet had positive correlation with the level of IL-2 、IFN-γ and had negative correlation with the level of IL-4、IL-10. The mRNA expressions of G