水稻Pi21基RNAi植株的抗稻瘟病研究及转mCherry基因水稻的遗传分析.pdfVIP

NormalMastermediatedmDissertaionPi21blastYa．HriceresistanceRNAiriceonandcourseofStudiesoftofor 研究工作在浙江省农业科学院“作物品质改良基因工程实验室’’ 完成 was ofScienceand Thiswork by financmHysupposedDepartment researchfor interest TechnologypubUc agriculturalprojects the researchfoundafionof (2010R21A52D03)andspecialized AcademyofAgricultural Zhengjinag ThisworkwasdoneinGenetic of EngineeringLaboratoryCrop Instituteof and QualityImprovement VirologyBiotechnology． T 摘要 只发生碱基缺失，说明左边界比右边界有更多的碱基变异类型。此外，对T2代植株 船丁转录水平因T-DNA在水稻基因组中的整合位置而变化，表现位置效应。本研究 在mCherry荧光蛋白和其它相关方法的基础上，为转基因水稻遗传及分子分析建立 了一个高效的流程体系。 关键词：水稻；稻瘟病；RNAi；pi21；mChet·ry；T-DNA II waswaswaswereworld．ItwithwithwithwaswerewithwithwerewereABCABCABSTRACTmostmainmediatedmCherwPi21．bothPi21blastbecomesblast．InusedPi21bhstPi21一RNAiblastPi21untransformedPi21-RNAiblastToTlaaahhPi21blastvectorareasanasTheRicericericericericericeresistanceresistance．riceresistancericerecessivericeresistancericeRNAiriceRT-PCRriceRNAiresistancericericerelatedRT-PCR．TheRNAireduced．AndRNAioneonevariationverticalvia48visualriceresistanceRNAiriceOsWRKY45onofalsoandand93andafterandafterandandafterandofofofofdisease-resistantoftenofofofofPi21ofofofofofofblast2tothefactorsfromforforfluorescentStudiesandandandandandanddiseasescultivationcultivarsdifferentiationcausecultivarsdiseascultivarsdurablediseasecloneddurable3’UTRdownstreamconstructioncallusdecreaseddiseasedifferentcontrolcontrolsofthethetheeffectivetothis7thetransformedthatthethetothetimethethoseestablishexaminationthetheIIIdimculttothetheirthethetheInseriousshowedshowedi